The University of Southampton
University of Southampton Institutional Repository

MMP-9/gelatinase B is a gene product of human adult articular chondrocytes and increased in osteoarthritic cartilage

Söder, S., Roach, H.I., Oehler, S., Bau, B., Haag, J. and Aigner, T. (2006) MMP-9/gelatinase B is a gene product of human adult articular chondrocytes and increased in osteoarthritic cartilage Clinical and Experimental Rheumatology, 24, (3), pp. 302-304.

Record type: Article


OBJECTIVE: Collagen fibril degeneration involves initially the cleavage within the triple helix by the collagenases 1 (MMP-1) and 3 (MMP-13), but then mainly involves also the gelatinases A (MMP-2) and B (MMP-9). The objective of this study was to determine the quantitative expression levels as well as the distribution in normal and osteoarthritic cartilage of gelatinase B and in cultured articular chondrocytes with and without stimulation by Il-1Beta.
METHODS: Conventional and real-time quantitative PCR technology and immunohistochemistry were used to determine gelatinase B expression on the mRNA and protein level.
RESULTS: Conventional PCR analysis could demonstrate the presence of gelatinase B mRNA only in osteoarthritic chondrocytes. Real-time quantitative PCR confirmed the increased expression of gelatinase B mRNA expression in osteoarthritic chondrocytes. No significant up-regulation of gelatinase B was observed by Il-1Beta. Immunostaining for gelatinase B showed the presence of gelatinase B in a subset of normal and in a large portion of osteoarthritic chondrocytes with a more extended distribution in the latter.
CONCLUSION: In osteoarthritic cartilage destruction, gelatinase B is involved in collagen destruction though still at a very much lower level than gelatinase A. Only a very small subset of normal adult articular chondrocytes express gelatinase B in vivo suggesting that gelatinase B unlike gelatinase A is hardly or only very focally involved in physiological collagen turnover.

Full text not available from this repository.

More information

Published date: May 2006
Keywords: immunohistochemistry, messenger, gene expression, middle aged, human, rna, cytology, reverse transcriptase polymerase chain reaction, gene, adult, osteoarthritis, genetics, expression, knee, collagen, cultured, chondrocytes, cartilage, pharmacology, protein, pathology, cells, drug, interleukin-1, aged, metabolism, analysis, articular, 80 and over, humans, drug effects, methods, research, in-vivo, femur head, biological markers, dose-response relationship, matrix metalloproteinase 9, up-regulation, germany


Local EPrints ID: 61530
ISSN: 0392-856X
PURE UUID: 4901e5eb-b378-4a98-89a8-0a88199c9dc7

Catalogue record

Date deposited: 16 Jun 2009
Last modified: 17 Jul 2017 14:21

Export record


Author: S. Söder
Author: H.I. Roach
Author: S. Oehler
Author: B. Bau
Author: J. Haag
Author: T. Aigner

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.