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MMP-9/gelatinase B is a gene product of human adult articular chondrocytes and increased in osteoarthritic cartilage

MMP-9/gelatinase B is a gene product of human adult articular chondrocytes and increased in osteoarthritic cartilage
MMP-9/gelatinase B is a gene product of human adult articular chondrocytes and increased in osteoarthritic cartilage
OBJECTIVE: Collagen fibril degeneration involves initially the cleavage within the triple helix by the collagenases 1 (MMP-1) and 3 (MMP-13), but then mainly involves also the gelatinases A (MMP-2) and B (MMP-9). The objective of this study was to determine the quantitative expression levels as well as the distribution in normal and osteoarthritic cartilage of gelatinase B and in cultured articular chondrocytes with and without stimulation by Il-1Beta.
METHODS: Conventional and real-time quantitative PCR technology and immunohistochemistry were used to determine gelatinase B expression on the mRNA and protein level.
RESULTS: Conventional PCR analysis could demonstrate the presence of gelatinase B mRNA only in osteoarthritic chondrocytes. Real-time quantitative PCR confirmed the increased expression of gelatinase B mRNA expression in osteoarthritic chondrocytes. No significant up-regulation of gelatinase B was observed by Il-1Beta. Immunostaining for gelatinase B showed the presence of gelatinase B in a subset of normal and in a large portion of osteoarthritic chondrocytes with a more extended distribution in the latter.
CONCLUSION: In osteoarthritic cartilage destruction, gelatinase B is involved in collagen destruction though still at a very much lower level than gelatinase A. Only a very small subset of normal adult articular chondrocytes express gelatinase B in vivo suggesting that gelatinase B unlike gelatinase A is hardly or only very focally involved in physiological collagen turnover.
immunohistochemistry, messenger, gene expression, middle aged, human, rna, cytology, reverse transcriptase polymerase chain reaction, gene, adult, osteoarthritis, genetics, expression, knee, collagen, cultured, chondrocytes, cartilage, pharmacology, protein, pathology, cells, drug, interleukin-1, aged, metabolism, analysis, articular, 80 and over, humans, drug effects, methods, research, in-vivo, femur head, biological markers, dose-response relationship, matrix metalloproteinase 9, up-regulation, germany
0392-856X
302-304
Söder, S.
a5a00be0-ce08-4508-9a15-f6a641369401
Roach, H.I.
1c0cf1f8-15dc-49a8-aad8-9ed3fd13c05b
Oehler, S.
d4de11ef-966d-44c3-a687-c77dad616f09
Bau, B.
77b90aba-36f5-41d1-b334-e7417fd15561
Haag, J.
fa1a489b-d536-4ed1-be94-650f71f6daaf
Aigner, T.
a735ee4e-077c-43ba-bea2-5dd31f957f87
Söder, S.
a5a00be0-ce08-4508-9a15-f6a641369401
Roach, H.I.
1c0cf1f8-15dc-49a8-aad8-9ed3fd13c05b
Oehler, S.
d4de11ef-966d-44c3-a687-c77dad616f09
Bau, B.
77b90aba-36f5-41d1-b334-e7417fd15561
Haag, J.
fa1a489b-d536-4ed1-be94-650f71f6daaf
Aigner, T.
a735ee4e-077c-43ba-bea2-5dd31f957f87

Söder, S., Roach, H.I., Oehler, S., Bau, B., Haag, J. and Aigner, T. (2006) MMP-9/gelatinase B is a gene product of human adult articular chondrocytes and increased in osteoarthritic cartilage. Clinical and Experimental Rheumatology, 24 (3), 302-304.

Record type: Article

Abstract

OBJECTIVE: Collagen fibril degeneration involves initially the cleavage within the triple helix by the collagenases 1 (MMP-1) and 3 (MMP-13), but then mainly involves also the gelatinases A (MMP-2) and B (MMP-9). The objective of this study was to determine the quantitative expression levels as well as the distribution in normal and osteoarthritic cartilage of gelatinase B and in cultured articular chondrocytes with and without stimulation by Il-1Beta.
METHODS: Conventional and real-time quantitative PCR technology and immunohistochemistry were used to determine gelatinase B expression on the mRNA and protein level.
RESULTS: Conventional PCR analysis could demonstrate the presence of gelatinase B mRNA only in osteoarthritic chondrocytes. Real-time quantitative PCR confirmed the increased expression of gelatinase B mRNA expression in osteoarthritic chondrocytes. No significant up-regulation of gelatinase B was observed by Il-1Beta. Immunostaining for gelatinase B showed the presence of gelatinase B in a subset of normal and in a large portion of osteoarthritic chondrocytes with a more extended distribution in the latter.
CONCLUSION: In osteoarthritic cartilage destruction, gelatinase B is involved in collagen destruction though still at a very much lower level than gelatinase A. Only a very small subset of normal adult articular chondrocytes express gelatinase B in vivo suggesting that gelatinase B unlike gelatinase A is hardly or only very focally involved in physiological collagen turnover.

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More information

Published date: May 2006
Keywords: immunohistochemistry, messenger, gene expression, middle aged, human, rna, cytology, reverse transcriptase polymerase chain reaction, gene, adult, osteoarthritis, genetics, expression, knee, collagen, cultured, chondrocytes, cartilage, pharmacology, protein, pathology, cells, drug, interleukin-1, aged, metabolism, analysis, articular, 80 and over, humans, drug effects, methods, research, in-vivo, femur head, biological markers, dose-response relationship, matrix metalloproteinase 9, up-regulation, germany

Identifiers

Local EPrints ID: 61530
URI: http://eprints.soton.ac.uk/id/eprint/61530
ISSN: 0392-856X
PURE UUID: 4901e5eb-b378-4a98-89a8-0a88199c9dc7

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Date deposited: 16 Jun 2009
Last modified: 22 Jul 2022 21:15

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Contributors

Author: S. Söder
Author: H.I. Roach
Author: S. Oehler
Author: B. Bau
Author: J. Haag
Author: T. Aigner

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