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Changes in immunolocalisation of ?-dystroglycan and specific degradative enzymes in the osteoarthritic synovium

Changes in immunolocalisation of ?-dystroglycan and specific degradative enzymes in the osteoarthritic synovium
Changes in immunolocalisation of ?-dystroglycan and specific degradative enzymes in the osteoarthritic synovium
OBJECTIVE: To investigate the immunolocalisation of ?-dystroglycan (?-DG) and specific matrix metalloproteinases (MMPs)-3, -9, -13 and a disintegrin like and metalloproteinase thrombospondin type 1 motif 4 (ADAMTS-4) within the joint tissues of patients with osteoarthritis (OA) and unaffected controls.
DESIGN: Cartilage, synovium and synovial fluid were obtained from the hip joints of five osteoarthritic (patients undergoing total hip replacement) and five control hip joints (patients undergoing hemiarthroplasty for femoral neck fracture). The samples were analysed for ?-DG protein using Western blot technique and by immunohistochemistry for tissue distribution of ?-DG, MMP-3, -9, -13, and ADAMTS-4.
RESULTS: ?-DG was detected in the smooth muscle of both normal and osteoarthritic synovial blood vessels. Importantly, ?-DG was detected in endothelium of blood vessels of OA synovium, but not in the control endothelium. In the endothelium of osteoarthritic synovial blood vessels, ?-DG co-localised with MMP-3 and -9. MMP-13 and ADAMTS-4 showed no endothelial staining, and only weak staining of the vascular smooth muscle was found. In contrast, we did not detect ?-DG protein in cartilage or synovial fluid.
CONCLUSIONS: ?-DG has been shown to have a role in angiogenesis, and our results demonstrate for the first time that there are clear differences in ?-DG staining between OA and control synovial blood vessels. The specific immunolocalisation of ?-DG within endothelium of inflamed OA blood vessels and its co-localisation with MMP-3 and -9, reported to have pro-angiogenic roles and believed to be involved in ?-DG cleavage, may also suggest that ?-DG plays a role in angiogenesis accompanying OA.
proteins, analysis, matrix metalloproteinase 3, articular, adam proteins, protein, hip joint, matrix metalloproteinase 13, osteoarthritis, humans, endothelium, methods, human, smooth, vascular, synovial membrane, blotting, hip, matrix metalloproteinases, metabolism, procollagen n-endopeptidase, enzymology, muscle, matrix metalloproteinase 9, synovial fluid, tissue distribution, cartilage, immunohistochemistry, time, procollagen, neck, dystroglycans, western, surgery, blood
1063-4584
1181-1188
Wimsey, S.
5f21fc9e-c1b0-47c7-b357-0821f0741bf0
Lien, C.F.
f82a654f-3679-44bf-849e-c5bb73202722
Sharma, S.
006485ea-336e-4389-b3ab-93d1484c0ca0
Brennan, P.A.
0b128e06-601c-4d40-97f8-3b10b3624378
Roach, H.I.
1c0cf1f8-15dc-49a8-aad8-9ed3fd13c05b
Harper, G.D.
d5ad1f9e-5899-4e2a-94a2-d38f76eb7c69
Górecki, D.C.
33ffe047-c6c8-4b36-864e-93c55d1c0a5a
Wimsey, S.
5f21fc9e-c1b0-47c7-b357-0821f0741bf0
Lien, C.F.
f82a654f-3679-44bf-849e-c5bb73202722
Sharma, S.
006485ea-336e-4389-b3ab-93d1484c0ca0
Brennan, P.A.
0b128e06-601c-4d40-97f8-3b10b3624378
Roach, H.I.
1c0cf1f8-15dc-49a8-aad8-9ed3fd13c05b
Harper, G.D.
d5ad1f9e-5899-4e2a-94a2-d38f76eb7c69
Górecki, D.C.
33ffe047-c6c8-4b36-864e-93c55d1c0a5a

Wimsey, S., Lien, C.F., Sharma, S., Brennan, P.A., Roach, H.I., Harper, G.D. and Górecki, D.C. (2006) Changes in immunolocalisation of ?-dystroglycan and specific degradative enzymes in the osteoarthritic synovium. Osteoarthritis and Cartilage, 14 (11), 1181-1188. (doi:10.1016/j.joca.2006.04.012).

Record type: Article

Abstract

OBJECTIVE: To investigate the immunolocalisation of ?-dystroglycan (?-DG) and specific matrix metalloproteinases (MMPs)-3, -9, -13 and a disintegrin like and metalloproteinase thrombospondin type 1 motif 4 (ADAMTS-4) within the joint tissues of patients with osteoarthritis (OA) and unaffected controls.
DESIGN: Cartilage, synovium and synovial fluid were obtained from the hip joints of five osteoarthritic (patients undergoing total hip replacement) and five control hip joints (patients undergoing hemiarthroplasty for femoral neck fracture). The samples were analysed for ?-DG protein using Western blot technique and by immunohistochemistry for tissue distribution of ?-DG, MMP-3, -9, -13, and ADAMTS-4.
RESULTS: ?-DG was detected in the smooth muscle of both normal and osteoarthritic synovial blood vessels. Importantly, ?-DG was detected in endothelium of blood vessels of OA synovium, but not in the control endothelium. In the endothelium of osteoarthritic synovial blood vessels, ?-DG co-localised with MMP-3 and -9. MMP-13 and ADAMTS-4 showed no endothelial staining, and only weak staining of the vascular smooth muscle was found. In contrast, we did not detect ?-DG protein in cartilage or synovial fluid.
CONCLUSIONS: ?-DG has been shown to have a role in angiogenesis, and our results demonstrate for the first time that there are clear differences in ?-DG staining between OA and control synovial blood vessels. The specific immunolocalisation of ?-DG within endothelium of inflamed OA blood vessels and its co-localisation with MMP-3 and -9, reported to have pro-angiogenic roles and believed to be involved in ?-DG cleavage, may also suggest that ?-DG plays a role in angiogenesis accompanying OA.

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More information

Published date: November 2006
Keywords: proteins, analysis, matrix metalloproteinase 3, articular, adam proteins, protein, hip joint, matrix metalloproteinase 13, osteoarthritis, humans, endothelium, methods, human, smooth, vascular, synovial membrane, blotting, hip, matrix metalloproteinases, metabolism, procollagen n-endopeptidase, enzymology, muscle, matrix metalloproteinase 9, synovial fluid, tissue distribution, cartilage, immunohistochemistry, time, procollagen, neck, dystroglycans, western, surgery, blood

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Local EPrints ID: 61613
URI: http://eprints.soton.ac.uk/id/eprint/61613
ISSN: 1063-4584
PURE UUID: ec72445b-a626-4a07-8a37-0540e04e694a

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Date deposited: 09 Sep 2008
Last modified: 15 Mar 2024 11:27

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Contributors

Author: S. Wimsey
Author: C.F. Lien
Author: S. Sharma
Author: P.A. Brennan
Author: H.I. Roach
Author: G.D. Harper
Author: D.C. Górecki

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