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The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells

The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells
The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells
RNA interference (RNAi) has been used in functional genomics and offers innovative approaches in the development of novel therapeutics. Human mesenchymal stem cells offer a unique cell source for tissue engineering/regeneration strategies. The current study examined the potential of small interfering RNAs (siRNA) against human peroxisome proliferator activated receptor gamma (PPARgamma) to suppress adipocyte differentiation (adipogenesis) in human preadipocytes and fetal-femur-derived mesenchymal cells. Adipogenesis was investigated using cellular and biochemical analysis. Transient transfection with PPARgamma-siRNA using a liposomal-based strategy resulted in a significant inhibition of adipogenesis in human preadipocytes and fetal-femur-derived mesenchymal cells, compared to controls (cell, liposomal and negative siRNA). The inhibitory effect of PPARgamma-siRNA was supported by testing human PPARgamma mRNA and adipogenic associated genes using reverse transcription polymerase chain reaction (RT-PCR) to adiponectin receptor 1 and 2 as well as examination of fatty acid binding protein 3 (FABP3) expression, an adipocyte-specific marker. The current studies indicate that PPARgamma-siRNA is a useful tool to study adipogenesis in human cells, with potential applications both therapeutic and in the elucidation of mesenchymal cell differentiation in the modulation of cell differentiation in human mesenchymal cells.
small interfering rna, peroxisome proliferator activated receptor gamma, human predipocytes, human mesenchymal cells, adipocyte differentiation, differentiation
0014-4827
1856-1864
Xu, Y.
30b17987-352d-4c0e-b58b-25c03dcd223d
Mirmalek-Sani, S.-H.
0ed7279f-3c1e-445e-beb7-d113d051a94d
Yang, X.
aa9fef47-6ada-44e9-9239-3109f37f1638
Zhang, J.
722d2564-f8ae-40f1-b1e1-07896b67a0d8
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Xu, Y.
30b17987-352d-4c0e-b58b-25c03dcd223d
Mirmalek-Sani, S.-H.
0ed7279f-3c1e-445e-beb7-d113d051a94d
Yang, X.
aa9fef47-6ada-44e9-9239-3109f37f1638
Zhang, J.
722d2564-f8ae-40f1-b1e1-07896b67a0d8
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Xu, Y., Mirmalek-Sani, S.-H., Yang, X., Zhang, J. and Oreffo, R.O.C. (2006) The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells. Experimental Cell Research, 312 (10), 1856-1864. (doi:10.1016/j.yexcr.2006.02.016).

Record type: Article

Abstract

RNA interference (RNAi) has been used in functional genomics and offers innovative approaches in the development of novel therapeutics. Human mesenchymal stem cells offer a unique cell source for tissue engineering/regeneration strategies. The current study examined the potential of small interfering RNAs (siRNA) against human peroxisome proliferator activated receptor gamma (PPARgamma) to suppress adipocyte differentiation (adipogenesis) in human preadipocytes and fetal-femur-derived mesenchymal cells. Adipogenesis was investigated using cellular and biochemical analysis. Transient transfection with PPARgamma-siRNA using a liposomal-based strategy resulted in a significant inhibition of adipogenesis in human preadipocytes and fetal-femur-derived mesenchymal cells, compared to controls (cell, liposomal and negative siRNA). The inhibitory effect of PPARgamma-siRNA was supported by testing human PPARgamma mRNA and adipogenic associated genes using reverse transcription polymerase chain reaction (RT-PCR) to adiponectin receptor 1 and 2 as well as examination of fatty acid binding protein 3 (FABP3) expression, an adipocyte-specific marker. The current studies indicate that PPARgamma-siRNA is a useful tool to study adipogenesis in human cells, with potential applications both therapeutic and in the elucidation of mesenchymal cell differentiation in the modulation of cell differentiation in human mesenchymal cells.

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More information

Published date: 10 June 2006
Keywords: small interfering rna, peroxisome proliferator activated receptor gamma, human predipocytes, human mesenchymal cells, adipocyte differentiation, differentiation

Identifiers

Local EPrints ID: 61619
URI: http://eprints.soton.ac.uk/id/eprint/61619
ISSN: 0014-4827
PURE UUID: 09fc1c77-fd4b-4d45-81b0-19e3484a38da
ORCID for R.O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 06 Oct 2008
Last modified: 16 Mar 2024 03:11

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Contributors

Author: Y. Xu
Author: S.-H. Mirmalek-Sani
Author: X. Yang
Author: J. Zhang
Author: R.O.C. Oreffo ORCID iD

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