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The phagocytic capacity of neurones

The phagocytic capacity of neurones
The phagocytic capacity of neurones
Phagocytosis is defined as the ingestion of particulates over 0.5 mu m in diameter and is associated with cells of the immune system such as macrophages or monocytes. Neurones are not generally recognized to be phagocytic. Using light, confocal, time-lapse and electron microscopy, we carried out a wide range of in-vitro and in-vivo experiments to examine the phagocytic capacity of different neuronal cell types. We demonstrated phagocytosis of material by neurones, including cell debris and synthetic particles up to 2.8 mu m in diameter. We showed phagocytosis in different neuronal types, and demonstrated that debris can be transported from neurite extremities to cell bodies and persist within neurones. Flow cytometry analysis demonstrated the lack of certain complement receptors on neurones but the presence of others, including integrin receptors known to mediate macrophage phagocytosis, indicating that a restricted set of phagocytosis receptors may mediate this process. Neuronal phagocytosis occurs in vitro and in vivo, and we propose that this is a more widespread and significant process than previously recognized. Neuronal phagocytosis may explain certain inclusions in neurones during disease, cell-to-cell spread of disease, neuronal death during disease progression and provide a potential mechanism for therapeutic intervention through the delivery of particulate drug carriers.
autophagy, cell-death, chick-embryo, axons, alzheimers-disease, system, brain, neurone, mechanism, delivery, repair, phagocytosis, disease, retrograde transport, in-vivo, immune system, in vivo, cells, macrophages, neurodegeneration, spinal-cord, inclusion bodies
0953-816X
2947-2955
Bowen, Samantha
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Ateh, Davidson D.
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Deinhardt, Katrin
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Bird, Margaret M.
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Price, Karen M.
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Baker, Cathy S.
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Robson, Joanna C.
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Swash, Michael
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Shamsuddin, Wassim
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Kawar, Shalini
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El-Tawil, Tariq
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Roos, Jesper
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Hoyle, Andrew
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Nickols, Carole D.
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Knowles, Charles H.
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Pullen, Anthony H.
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Luthert, Philip J.
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Weller, Roy O.
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Hafezparast, Majid
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Franklin, Robin J.M.
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Revesz, Tamas
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King, Rosalind H.M.
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Berninghausen, Otto
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Fisher, Elizabeth M.C.
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Schiavo, Giampietro
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Martin, Joanne E.
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Bowen, Samantha
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Ateh, Davidson D.
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Deinhardt, Katrin
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Bird, Margaret M.
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Price, Karen M.
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Baker, Cathy S.
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Robson, Joanna C.
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Swash, Michael
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Shamsuddin, Wassim
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Kawar, Shalini
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El-Tawil, Tariq
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Roos, Jesper
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Hoyle, Andrew
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Nickols, Carole D.
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Knowles, Charles H.
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Pullen, Anthony H.
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Luthert, Philip J.
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Weller, Roy O.
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Hafezparast, Majid
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Franklin, Robin J.M.
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Revesz, Tamas
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King, Rosalind H.M.
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Berninghausen, Otto
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Fisher, Elizabeth M.C.
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Schiavo, Giampietro
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Martin, Joanne E.
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Bowen, Samantha, Ateh, Davidson D., Deinhardt, Katrin, Bird, Margaret M., Price, Karen M., Baker, Cathy S., Robson, Joanna C., Swash, Michael, Shamsuddin, Wassim, Kawar, Shalini, El-Tawil, Tariq, Roos, Jesper, Hoyle, Andrew, Nickols, Carole D., Knowles, Charles H., Pullen, Anthony H., Luthert, Philip J., Weller, Roy O., Hafezparast, Majid, Franklin, Robin J.M., Revesz, Tamas, King, Rosalind H.M., Berninghausen, Otto, Fisher, Elizabeth M.C., Schiavo, Giampietro and Martin, Joanne E. (2007) The phagocytic capacity of neurones. European Journal of Neuroscience, 25 (10), 2947-2955. (doi:10.1111/j.1460-9568.2007.05554.x).

Record type: Article

Abstract

Phagocytosis is defined as the ingestion of particulates over 0.5 mu m in diameter and is associated with cells of the immune system such as macrophages or monocytes. Neurones are not generally recognized to be phagocytic. Using light, confocal, time-lapse and electron microscopy, we carried out a wide range of in-vitro and in-vivo experiments to examine the phagocytic capacity of different neuronal cell types. We demonstrated phagocytosis of material by neurones, including cell debris and synthetic particles up to 2.8 mu m in diameter. We showed phagocytosis in different neuronal types, and demonstrated that debris can be transported from neurite extremities to cell bodies and persist within neurones. Flow cytometry analysis demonstrated the lack of certain complement receptors on neurones but the presence of others, including integrin receptors known to mediate macrophage phagocytosis, indicating that a restricted set of phagocytosis receptors may mediate this process. Neuronal phagocytosis occurs in vitro and in vivo, and we propose that this is a more widespread and significant process than previously recognized. Neuronal phagocytosis may explain certain inclusions in neurones during disease, cell-to-cell spread of disease, neuronal death during disease progression and provide a potential mechanism for therapeutic intervention through the delivery of particulate drug carriers.

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More information

Published date: 2007
Keywords: autophagy, cell-death, chick-embryo, axons, alzheimers-disease, system, brain, neurone, mechanism, delivery, repair, phagocytosis, disease, retrograde transport, in-vivo, immune system, in vivo, cells, macrophages, neurodegeneration, spinal-cord, inclusion bodies

Identifiers

Local EPrints ID: 62333
URI: https://eprints.soton.ac.uk/id/eprint/62333
ISSN: 0953-816X
PURE UUID: 3ad257cc-1af7-4102-8867-21a72a74a17a

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Date deposited: 12 Sep 2008
Last modified: 13 Mar 2019 20:27

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Contributors

Author: Samantha Bowen
Author: Davidson D. Ateh
Author: Katrin Deinhardt
Author: Margaret M. Bird
Author: Karen M. Price
Author: Cathy S. Baker
Author: Joanna C. Robson
Author: Michael Swash
Author: Wassim Shamsuddin
Author: Shalini Kawar
Author: Tariq El-Tawil
Author: Jesper Roos
Author: Andrew Hoyle
Author: Carole D. Nickols
Author: Charles H. Knowles
Author: Anthony H. Pullen
Author: Philip J. Luthert
Author: Roy O. Weller
Author: Majid Hafezparast
Author: Robin J.M. Franklin
Author: Tamas Revesz
Author: Rosalind H.M. King
Author: Otto Berninghausen
Author: Elizabeth M.C. Fisher
Author: Giampietro Schiavo
Author: Joanne E. Martin

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