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Leukocytes-depleting filters preferentially remove activated leukocytes and reduce the expression of surface adhesion molecules during the simulated extracorporeal circulation of human blood

Leukocytes-depleting filters preferentially remove activated leukocytes and reduce the expression of surface adhesion molecules during the simulated extracorporeal circulation of human blood
Leukocytes-depleting filters preferentially remove activated leukocytes and reduce the expression of surface adhesion molecules during the simulated extracorporeal circulation of human blood
The effect of leukocyte-depleting filters on the total and activated leukocyte counts and the expression of surface adhesion molecules CD11b, CD18, and CD62L during the in vitro extracorporeal circulation of human blood was studied. A 200 ml blood sample was taken from 10 patients undergoing CABG surgery. The blood was circulated for 60 minutes within an experimental extracorporeal circuit. A leukocyte-depleting filter was attached in five circuits (filtered group). In five other circuits, no filter was used (controls). Total leukocyte counts were determined manually. Activated leukocytes were identified using nitroblue tetrazolium staining. The expression of CD11b, CD18, and CD62L was measured with flow cytometry. At 60 minutes, total leukocyte counts were reduced by 49% from the baseline values in the filtered group and 10% in the control group (p < 0.0001). Activated leukocyte counts decreased by 86% in the filtered group and increased by 116% in the control group (p < 0.0001). In the filtered group, the expression of CD11b, CD18, and CD612L decreased by 60%, 21%, and 79%, respectively, and in the control group it increased by 24%, 6%, and 28% (p < 0.0001). Leukocyte-depleting filters preferentially remove activated leukocytes and reduce the expression of CD11b, CD18, and CD62L during the in vitro extracorporeal circulation of human blood
surgery, expression, patient, cardiopulmonary bypass, in-vitro, leucodepletion, filtration, molecules, neutrophils, white cell-reduction, surface, sample, injury, time, coronary revascularization, granulocytes
1058-2916
438-444
Alexiou, C.
cb234ab6-eef6-4366-be16-803c0dbc9d0c
Sheppard, S.
f08f09bc-286a-4470-81d7-2727a7a4471a
Tang, A.
5cdc08d6-3fbe-433a-8199-a075b31a39f6
Rengarajan, A.
f27a4622-bdb8-4dab-a961-3e0fb8cf59d6
Smith, D.
217b19f1-7588-4d54-a062-8394eea6dea1
Haw, M.
05572348-43ba-4539-a713-0837ab2c3cee
Gibbs, R.
6e0629be-87c9-4daa-a95b-df78c7e543dd
Alexiou, C.
cb234ab6-eef6-4366-be16-803c0dbc9d0c
Sheppard, S.
f08f09bc-286a-4470-81d7-2727a7a4471a
Tang, A.
5cdc08d6-3fbe-433a-8199-a075b31a39f6
Rengarajan, A.
f27a4622-bdb8-4dab-a961-3e0fb8cf59d6
Smith, D.
217b19f1-7588-4d54-a062-8394eea6dea1
Haw, M.
05572348-43ba-4539-a713-0837ab2c3cee
Gibbs, R.
6e0629be-87c9-4daa-a95b-df78c7e543dd

Alexiou, C., Sheppard, S., Tang, A., Rengarajan, A., Smith, D., Haw, M. and Gibbs, R. (2006) Leukocytes-depleting filters preferentially remove activated leukocytes and reduce the expression of surface adhesion molecules during the simulated extracorporeal circulation of human blood. Asaio Journal, 52 (4), 438-444.

Record type: Article

Abstract

The effect of leukocyte-depleting filters on the total and activated leukocyte counts and the expression of surface adhesion molecules CD11b, CD18, and CD62L during the in vitro extracorporeal circulation of human blood was studied. A 200 ml blood sample was taken from 10 patients undergoing CABG surgery. The blood was circulated for 60 minutes within an experimental extracorporeal circuit. A leukocyte-depleting filter was attached in five circuits (filtered group). In five other circuits, no filter was used (controls). Total leukocyte counts were determined manually. Activated leukocytes were identified using nitroblue tetrazolium staining. The expression of CD11b, CD18, and CD62L was measured with flow cytometry. At 60 minutes, total leukocyte counts were reduced by 49% from the baseline values in the filtered group and 10% in the control group (p < 0.0001). Activated leukocyte counts decreased by 86% in the filtered group and increased by 116% in the control group (p < 0.0001). In the filtered group, the expression of CD11b, CD18, and CD612L decreased by 60%, 21%, and 79%, respectively, and in the control group it increased by 24%, 6%, and 28% (p < 0.0001). Leukocyte-depleting filters preferentially remove activated leukocytes and reduce the expression of CD11b, CD18, and CD62L during the in vitro extracorporeal circulation of human blood

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More information

Published date: 2006
Keywords: surgery, expression, patient, cardiopulmonary bypass, in-vitro, leucodepletion, filtration, molecules, neutrophils, white cell-reduction, surface, sample, injury, time, coronary revascularization, granulocytes

Identifiers

Local EPrints ID: 62667
URI: http://eprints.soton.ac.uk/id/eprint/62667
ISSN: 1058-2916
PURE UUID: 6118b243-e794-4e50-b745-04a1df90402e

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Date deposited: 05 Sep 2008
Last modified: 13 Mar 2019 20:27

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