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A multicenter evaluation of comprehensive analysis of MLL translocations and fusion gene partners in acute leukemia using the MLL FusionChip device

A multicenter evaluation of comprehensive analysis of MLL translocations and fusion gene partners in acute leukemia using the MLL FusionChip device
A multicenter evaluation of comprehensive analysis of MLL translocations and fusion gene partners in acute leukemia using the MLL FusionChip device
Rearrangements of the MLL gene are significant in acute leukemia. Among the most frequent translocations are t(4;11)(q21;q23) and t(9;11)(p22;q23), which give rise to the MLL-AFF1 and MLL-MLLT3 fusion genes (alias MLL-AF4 and MLL-AF9) in acute lymphoblastic and acute myeloid leukemia, respectively. Current evidence suggests that determining the MLL status of acute leukemia, including precise identification of the partner gene, is important in defining appropriate treatment. This underscores the need for accurate detection methods. A novel molecular diagnostic device, the MLL FusionChip, has been successfully used to identify MLL fusion gene translocations in acute leukemia, including the precise breakpoint location. This study evaluated the performance of the MLL FusionChip within a routine clinical environment, comprising nine centers worldwide, in the analysis of 21 control and 136 patient samples. It was shown that the assay allowed accurate detection of the MLL fusion gene, regardless of the breakpoint location, and confirmed that this multiplex approach was robust in a global multicenter trial. The MLL FusionChip was shown to be superior to other detection methods. The type of molecular information provided by MLL FusionChip gave an indication of the appropriate primers to design for disease monitoring of MLL patients following treatment.
fusion gene, cytogenetics, children, rearrangements, workshop, myeloid-leukemia, abnormalities, aberrations, women, translocation, identification, genes, polymerase-chain-reaction, patient, hematological malignancies, disease, gene, time, transcripts, trial, fusion, leukemia, 11q23, multicenter, acute lymphoblastic-leukemia
0165-4608
17-22
Harrison, Christine J.
52da7673-509c-4b88-b92e-0c021c9c7d3e
Griffiths, Mike
f147af96-79ab-43ef-984c-550407b82c39
Moorman, Fiona
24cf11b5-f7ae-4071-94e3-d098fa659854
Schnittger, Susanne
5157942c-96eb-47cd-9837-3feea8ec8984
Cayuela, Jean-Michel
006c25d4-e8b6-4e21-82a1-10702e055c72
Shurtleff, Sheila
55106b78-29c6-438a-a48c-1eb338b97f03
Gottardi, Enrico
94515fff-ef5b-483c-ad0d-97c5c68d5a00
Mitterbauer, Gerlinde
30c5afef-e726-4825-b8a5-16ba2afa47c4
Colomer, Dolores
d8c119b0-5523-4a57-bb9b-07836825dd22
Delabesse, Eric
3e410e8b-6d93-40cf-a7d5-5cd30a7f856d
Casteras, Vincent
0a082158-afe8-4aa6-b1cd-7831593a19e5
Harrison, Christine J.
52da7673-509c-4b88-b92e-0c021c9c7d3e
Griffiths, Mike
f147af96-79ab-43ef-984c-550407b82c39
Moorman, Fiona
24cf11b5-f7ae-4071-94e3-d098fa659854
Schnittger, Susanne
5157942c-96eb-47cd-9837-3feea8ec8984
Cayuela, Jean-Michel
006c25d4-e8b6-4e21-82a1-10702e055c72
Shurtleff, Sheila
55106b78-29c6-438a-a48c-1eb338b97f03
Gottardi, Enrico
94515fff-ef5b-483c-ad0d-97c5c68d5a00
Mitterbauer, Gerlinde
30c5afef-e726-4825-b8a5-16ba2afa47c4
Colomer, Dolores
d8c119b0-5523-4a57-bb9b-07836825dd22
Delabesse, Eric
3e410e8b-6d93-40cf-a7d5-5cd30a7f856d
Casteras, Vincent
0a082158-afe8-4aa6-b1cd-7831593a19e5

Harrison, Christine J., Griffiths, Mike, Moorman, Fiona, Schnittger, Susanne, Cayuela, Jean-Michel, Shurtleff, Sheila, Gottardi, Enrico, Mitterbauer, Gerlinde, Colomer, Dolores, Delabesse, Eric and Casteras, Vincent (2007) A multicenter evaluation of comprehensive analysis of MLL translocations and fusion gene partners in acute leukemia using the MLL FusionChip device. Cancer Genetics and Cytogenetics, 173 (1), 17-22. (doi:10.1016/j.cancergencyto.2006.09.006).

Record type: Article

Abstract

Rearrangements of the MLL gene are significant in acute leukemia. Among the most frequent translocations are t(4;11)(q21;q23) and t(9;11)(p22;q23), which give rise to the MLL-AFF1 and MLL-MLLT3 fusion genes (alias MLL-AF4 and MLL-AF9) in acute lymphoblastic and acute myeloid leukemia, respectively. Current evidence suggests that determining the MLL status of acute leukemia, including precise identification of the partner gene, is important in defining appropriate treatment. This underscores the need for accurate detection methods. A novel molecular diagnostic device, the MLL FusionChip, has been successfully used to identify MLL fusion gene translocations in acute leukemia, including the precise breakpoint location. This study evaluated the performance of the MLL FusionChip within a routine clinical environment, comprising nine centers worldwide, in the analysis of 21 control and 136 patient samples. It was shown that the assay allowed accurate detection of the MLL fusion gene, regardless of the breakpoint location, and confirmed that this multiplex approach was robust in a global multicenter trial. The MLL FusionChip was shown to be superior to other detection methods. The type of molecular information provided by MLL FusionChip gave an indication of the appropriate primers to design for disease monitoring of MLL patients following treatment.

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More information

Published date: February 2007
Keywords: fusion gene, cytogenetics, children, rearrangements, workshop, myeloid-leukemia, abnormalities, aberrations, women, translocation, identification, genes, polymerase-chain-reaction, patient, hematological malignancies, disease, gene, time, transcripts, trial, fusion, leukemia, 11q23, multicenter, acute lymphoblastic-leukemia

Identifiers

Local EPrints ID: 62782
URI: http://eprints.soton.ac.uk/id/eprint/62782
ISSN: 0165-4608
PURE UUID: 8e297195-e41d-4fdf-b46d-b0c3dd58f284

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Date deposited: 04 Sep 2008
Last modified: 15 Mar 2024 11:32

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Contributors

Author: Christine J. Harrison
Author: Mike Griffiths
Author: Fiona Moorman
Author: Susanne Schnittger
Author: Jean-Michel Cayuela
Author: Sheila Shurtleff
Author: Enrico Gottardi
Author: Gerlinde Mitterbauer
Author: Dolores Colomer
Author: Eric Delabesse
Author: Vincent Casteras

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