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Priming protective CD8 T cell immunity by DNA vaccines encoding chimeric, stress protein-capturing tumor-associated antigen

Priming protective CD8 T cell immunity by DNA vaccines encoding chimeric, stress protein-capturing tumor-associated antigen
Priming protective CD8 T cell immunity by DNA vaccines encoding chimeric, stress protein-capturing tumor-associated antigen
DNA vaccines encoding heat shock protein (hsp)-capturing, chimeric peptides containing antigenic determinants of the tumor-associated Ag (TAA) gp70 (an envelope protein of endogenous retrovirus) primed stable, specific, and tumor-protective CD8 T cell immunity. Expression of gp70 transcripts was detectable in most normal tissues but was particularly striking in some (but not all) tumor cell lines tested (including the adenocarcinoma cell line CT26). An similar to 200 residue gp70 fragment or its L-d-binding antigenic AH1 peptide cloned in-frame behind an hsp-capturing (cT(272)) or noncapturing (T-60) N-terminal large SV40 tumor Ag sequence was expressed as either hsp-binding or -nonbinding chimeric Ags. Only hsp-capturing, chimeric fusion proteins were expressed efficiently in transfected cell lines and primed TAA-specific CD8 T cell immunity. This immunity mediated protection in the CT26 and mKSA models. A vaccination strategy based on delivering antigenic, hsp-associated TAA fragments can thus prime protective CD8 T cell immunity even if these TAA are of low intrinsic immunogenicity.
models, time, cell, virus env gene, transcripts, fusion, t-cell, model, heat shock protein, fusion proteins, protein, tissue, endogenous retrovirus, peptide, tumor, dendritic cells, immunity, vaccine, class-i molecules, therapeutic antitumor immunity, heat-shock proteins, determinants, mammary adenocarcinoma, colon-tumor, adenocarcinoma, dna, expression, antigen
0022-1767
1534-1542
Schirmbeck, Reinhold
ec51d4e6-c2dc-427c-a295-91340555570c
Riedl, Petra
afe9702f-620c-4de7-8d78-e6f73d78bdce
Kupferschmitt, Mark
b5461032-16a5-4603-9094-0613aceec5ce
Wegenka, Ursula
3c508c27-e3b5-449b-9798-17601523780b
Hauser, Hansjörg
5f4fe4b5-5a88-4f23-b636-5cf32669bcac
Rice, Jason
d58d4fcd-8dc0-4599-bf96-62323d579227
Kroger, Andrea
7a0c33d7-ad70-4185-b12e-64d07eeb3eb7
Reimann, Jörg
9c893c4a-7f01-48cf-af35-db27c0599e81
Schirmbeck, Reinhold
ec51d4e6-c2dc-427c-a295-91340555570c
Riedl, Petra
afe9702f-620c-4de7-8d78-e6f73d78bdce
Kupferschmitt, Mark
b5461032-16a5-4603-9094-0613aceec5ce
Wegenka, Ursula
3c508c27-e3b5-449b-9798-17601523780b
Hauser, Hansjörg
5f4fe4b5-5a88-4f23-b636-5cf32669bcac
Rice, Jason
d58d4fcd-8dc0-4599-bf96-62323d579227
Kroger, Andrea
7a0c33d7-ad70-4185-b12e-64d07eeb3eb7
Reimann, Jörg
9c893c4a-7f01-48cf-af35-db27c0599e81

Schirmbeck, Reinhold, Riedl, Petra, Kupferschmitt, Mark, Wegenka, Ursula, Hauser, Hansjörg, Rice, Jason, Kroger, Andrea and Reimann, Jörg (2006) Priming protective CD8 T cell immunity by DNA vaccines encoding chimeric, stress protein-capturing tumor-associated antigen. The Journal of Immunology, 177 (3), 1534-1542.

Record type: Article

Abstract

DNA vaccines encoding heat shock protein (hsp)-capturing, chimeric peptides containing antigenic determinants of the tumor-associated Ag (TAA) gp70 (an envelope protein of endogenous retrovirus) primed stable, specific, and tumor-protective CD8 T cell immunity. Expression of gp70 transcripts was detectable in most normal tissues but was particularly striking in some (but not all) tumor cell lines tested (including the adenocarcinoma cell line CT26). An similar to 200 residue gp70 fragment or its L-d-binding antigenic AH1 peptide cloned in-frame behind an hsp-capturing (cT(272)) or noncapturing (T-60) N-terminal large SV40 tumor Ag sequence was expressed as either hsp-binding or -nonbinding chimeric Ags. Only hsp-capturing, chimeric fusion proteins were expressed efficiently in transfected cell lines and primed TAA-specific CD8 T cell immunity. This immunity mediated protection in the CT26 and mKSA models. A vaccination strategy based on delivering antigenic, hsp-associated TAA fragments can thus prime protective CD8 T cell immunity even if these TAA are of low intrinsic immunogenicity.

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More information

Published date: 2006
Keywords: models, time, cell, virus env gene, transcripts, fusion, t-cell, model, heat shock protein, fusion proteins, protein, tissue, endogenous retrovirus, peptide, tumor, dendritic cells, immunity, vaccine, class-i molecules, therapeutic antitumor immunity, heat-shock proteins, determinants, mammary adenocarcinoma, colon-tumor, adenocarcinoma, dna, expression, antigen

Identifiers

Local EPrints ID: 62919
URI: http://eprints.soton.ac.uk/id/eprint/62919
ISSN: 0022-1767
PURE UUID: c98fef48-9d36-4e5e-a242-7ed59e33e064

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Date deposited: 05 Sep 2008
Last modified: 13 Mar 2019 20:26

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Contributors

Author: Reinhold Schirmbeck
Author: Petra Riedl
Author: Mark Kupferschmitt
Author: Ursula Wegenka
Author: Hansjörg Hauser
Author: Jason Rice
Author: Andrea Kroger
Author: Jörg Reimann

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