The University of Southampton
University of Southampton Institutional Repository

Requirement for CD70 in CD4(+) Th cell-dependent and innate receptor-mediated CD8(+) T cell priming

Requirement for CD70 in CD4(+) Th cell-dependent and innate receptor-mediated CD8(+) T cell priming
Requirement for CD70 in CD4(+) Th cell-dependent and innate receptor-mediated CD8(+) T cell priming
Dendritic cell (DC) conditioning by CD4(+) T cells, or via engagement of innate receptors, is thought to be essential for CD8(+) T cell priming. However, the molecular features that distinguish a conditioned DC from an unconditioned DC are poorly defined. In this study. we investigate the role of CD70, a member of the TNF superfamily that is expressed on activated DC, in CD4+ Th-dependent and -independent CD8(+) T cell responses. We demonstrate that CD70 is required for CD4(+) T cell-dependent priming of CD8(+) T cells as well as priming mediated by the viral signature, dsRNA. Accordingly, mice that were subjected to CD70 blockade during the primary response fail to generate a memory CD8(+) T cell response. Furthermore, we find that CD70 is dispensable for CD4(+) T cell expansion and help for B cells, thus suggesting a direct role for CD70 in CD8(+) T cell priming. Our results show that the innate and adaptive (CD4(+) T cells) arms of the immune system use a common signaling pathway in driving CD8(+) T cell responses and suggest that expression of CD70 on DC represents the hallmark of conditioned DC.
cd70, dendritic cells, expression, toll-like receptor-3, double-stranded-rna, responses in-vivo, viral-infection, responses, cd4-t-cell help, clonal expansion, antigen-presenting cells, cd8-t-cell memory, t-cells, cutting edge, innate
0022-1767
2969-2975
Taraban, Vadim Y.
d709dcbb-ff36-4514-85da-08ca68aafcd3
Rowley, Tania F.
3a29866f-3e2c-4ee8-8fe6-fd189ac1eb3d
Tough, David F.
d1f1b88c-eaaf-45d6-ab2e-1bf74c52b41f
Al-Shamkhani, Aymen
0a40b3ce-9d71-4d41-9369-7212f0a84504
Taraban, Vadim Y.
d709dcbb-ff36-4514-85da-08ca68aafcd3
Rowley, Tania F.
3a29866f-3e2c-4ee8-8fe6-fd189ac1eb3d
Tough, David F.
d1f1b88c-eaaf-45d6-ab2e-1bf74c52b41f
Al-Shamkhani, Aymen
0a40b3ce-9d71-4d41-9369-7212f0a84504

Taraban, Vadim Y., Rowley, Tania F., Tough, David F. and Al-Shamkhani, Aymen (2006) Requirement for CD70 in CD4(+) Th cell-dependent and innate receptor-mediated CD8(+) T cell priming. The Journal of Immunology, 177 (5), 2969-2975.

Record type: Article

Abstract

Dendritic cell (DC) conditioning by CD4(+) T cells, or via engagement of innate receptors, is thought to be essential for CD8(+) T cell priming. However, the molecular features that distinguish a conditioned DC from an unconditioned DC are poorly defined. In this study. we investigate the role of CD70, a member of the TNF superfamily that is expressed on activated DC, in CD4+ Th-dependent and -independent CD8(+) T cell responses. We demonstrate that CD70 is required for CD4(+) T cell-dependent priming of CD8(+) T cells as well as priming mediated by the viral signature, dsRNA. Accordingly, mice that were subjected to CD70 blockade during the primary response fail to generate a memory CD8(+) T cell response. Furthermore, we find that CD70 is dispensable for CD4(+) T cell expansion and help for B cells, thus suggesting a direct role for CD70 in CD8(+) T cell priming. Our results show that the innate and adaptive (CD4(+) T cells) arms of the immune system use a common signaling pathway in driving CD8(+) T cell responses and suggest that expression of CD70 on DC represents the hallmark of conditioned DC.

Full text not available from this repository.

More information

Published date: 2006
Keywords: cd70, dendritic cells, expression, toll-like receptor-3, double-stranded-rna, responses in-vivo, viral-infection, responses, cd4-t-cell help, clonal expansion, antigen-presenting cells, cd8-t-cell memory, t-cells, cutting edge, innate

Identifiers

Local EPrints ID: 62945
URI: http://eprints.soton.ac.uk/id/eprint/62945
ISSN: 0022-1767
PURE UUID: e31fd023-1da7-4b76-a2f2-abe6924663a9
ORCID for Aymen Al-Shamkhani: ORCID iD orcid.org/0000-0003-0727-4189

Catalogue record

Date deposited: 05 Sep 2008
Last modified: 17 Dec 2019 01:54

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×