The University of Southampton
University of Southampton Institutional Repository

Assaying picoplankton distribution by flow cytometry of underway samples collected along a meridional transect across the Atlantic Ocean

Assaying picoplankton distribution by flow cytometry of underway samples collected along a meridional transect across the Atlantic Ocean
Assaying picoplankton distribution by flow cytometry of underway samples collected along a meridional transect across the Atlantic Ocean
The composition of picoplankton in near-surface samples collected underway at least every 6 h from a ship sailing from the Falkland Islands to the British Isles was determined by flow cytometry, using both autofluorescence markers and staining with the DNA dye SYBR Green I. Prochlorococcus (0.1 to 0.3 X 106 cells ml-l) dominated the phytoplankton in the oligotrophic northern (24" to 38" N) and southern (8" to 34" S) gyre regions. In the equatorial region (8" S to 24" N) the more productive waters supported 10 to 100 times more picoeukaryote algae and Synechococcus than in the gyres. The collection of samples underway proved to be a very economical method of obtaining a good indication of the concentration of heterotroph~c bactena and of these 3 classes of picophytoplankton in the surface mixed layer (coefficient of correlation, r2 = 0.88 to 0.98) throughout a very long transect, but correlated less well with picoplankton standing stocks in the top 200 m of the water column (r2 = 0.39 to 0.88). Daily variations in the flow cytometry profiles of stained Prochlorococcus observed over a distance of some 8000 km of the tropical and subtropical Atlantic were interpreted as an indication of their synchronised cell division in surface waters. Their estimated average minimum growth rate (0.15 d-l) was similar in both southern and northern oligotrophic gyres and in equatorial waters.
0948-3055
13-20
Zubkov, Mikhail V.
b1dfb3a0-bcff-430c-9031-358a22b50743
Sleigh, Michael A.
56f48166-32e6-4bc8-bc02-bc0667280989
Burkill, Peter H.
511b68df-1664-418f-baa1-b1075f1e1fed
Zubkov, Mikhail V.
b1dfb3a0-bcff-430c-9031-358a22b50743
Sleigh, Michael A.
56f48166-32e6-4bc8-bc02-bc0667280989
Burkill, Peter H.
511b68df-1664-418f-baa1-b1075f1e1fed

Zubkov, Mikhail V., Sleigh, Michael A. and Burkill, Peter H. (2000) Assaying picoplankton distribution by flow cytometry of underway samples collected along a meridional transect across the Atlantic Ocean. Aquatic Microbial Ecology, 21 (1), 13-20. (doi:10.3354/ame021013).

Record type: Article

Abstract

The composition of picoplankton in near-surface samples collected underway at least every 6 h from a ship sailing from the Falkland Islands to the British Isles was determined by flow cytometry, using both autofluorescence markers and staining with the DNA dye SYBR Green I. Prochlorococcus (0.1 to 0.3 X 106 cells ml-l) dominated the phytoplankton in the oligotrophic northern (24" to 38" N) and southern (8" to 34" S) gyre regions. In the equatorial region (8" S to 24" N) the more productive waters supported 10 to 100 times more picoeukaryote algae and Synechococcus than in the gyres. The collection of samples underway proved to be a very economical method of obtaining a good indication of the concentration of heterotroph~c bactena and of these 3 classes of picophytoplankton in the surface mixed layer (coefficient of correlation, r2 = 0.88 to 0.98) throughout a very long transect, but correlated less well with picoplankton standing stocks in the top 200 m of the water column (r2 = 0.39 to 0.88). Daily variations in the flow cytometry profiles of stained Prochlorococcus observed over a distance of some 8000 km of the tropical and subtropical Atlantic were interpreted as an indication of their synchronised cell division in surface waters. Their estimated average minimum growth rate (0.15 d-l) was similar in both southern and northern oligotrophic gyres and in equatorial waters.

This record has no associated files available for download.

More information

Published date: 2000

Identifiers

Local EPrints ID: 63152
URI: http://eprints.soton.ac.uk/id/eprint/63152
ISSN: 0948-3055
PURE UUID: cb20dcb4-db06-473a-9d4e-5e171ab6a1e1

Catalogue record

Date deposited: 15 Sep 2008
Last modified: 15 Mar 2024 11:36

Export record

Altmetrics

Contributors

Author: Mikhail V. Zubkov
Author: Michael A. Sleigh
Author: Peter H. Burkill

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×