An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide
An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide
A new acoustic-optical-microfluidic system is presented for the manipulation of bead-tagged DNA molecules. Acoustic radiation forces are used to manipulate microspheres into and away from the evanescent field of a laser coupled waveguide that is integrated into the reflector of the acoustic chamber. With suitable fluorophores the presence of the target DNA can be detected with a fluorescence microscope enabling large populations of beads to be examined simultaneously. The integrated waveguide and multimodal acoustic chamber are presented here, with results showing that the microspheres can be successfully detected as they are brought into the evanescent field using a quarter-wave acoustic configuration. It is also shown that by measuring the time of flight of a microsphere between the half- and quarterwave nodal planes the bead size can be determined, providing a means of multiplexing the detection (detecting a range of different target DNA sequences).
p.3925
Glynne-Jones, Peter
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Hill, Martyn
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Townsend, Rosemary J.
f40068ec-6cf6-4896-827a-379b600b032b
Harris, Nicholas R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Zhang, Fan
22a0ac7a-2068-4eef-a441-c8efb8f24ed0
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
May 2008
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Townsend, Rosemary J.
f40068ec-6cf6-4896-827a-379b600b032b
Harris, Nicholas R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Zhang, Fan
22a0ac7a-2068-4eef-a441-c8efb8f24ed0
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Glynne-Jones, Peter, Hill, Martyn, Townsend, Rosemary J., Harris, Nicholas R., Wilkinson, James S., Zhang, Fan and Melvin, Tracy
(2008)
An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide.
Journal of the Acoustical Society of America, 123 (5), .
(doi:10.1121/1.2935963).
Abstract
A new acoustic-optical-microfluidic system is presented for the manipulation of bead-tagged DNA molecules. Acoustic radiation forces are used to manipulate microspheres into and away from the evanescent field of a laser coupled waveguide that is integrated into the reflector of the acoustic chamber. With suitable fluorophores the presence of the target DNA can be detected with a fluorescence microscope enabling large populations of beads to be examined simultaneously. The integrated waveguide and multimodal acoustic chamber are presented here, with results showing that the microspheres can be successfully detected as they are brought into the evanescent field using a quarter-wave acoustic configuration. It is also shown that by measuring the time of flight of a microsphere between the half- and quarterwave nodal planes the bead size can be determined, providing a means of multiplexing the detection (detecting a range of different target DNA sequences).
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Published date: May 2008
Additional Information:
(Paper 5pPAa2 at Acoustics '08, 4 July, Paris )
Identifiers
Local EPrints ID: 63332
URI: http://eprints.soton.ac.uk/id/eprint/63332
ISSN: 0001-4966
PURE UUID: 2c4f8b75-19d9-4450-a461-b4495d23895d
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Date deposited: 08 Oct 2008
Last modified: 07 Dec 2024 02:37
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Contributors
Author:
Rosemary J. Townsend
Author:
Nicholas R. Harris
Author:
Fan Zhang
Author:
Tracy Melvin
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