The University of Southampton
University of Southampton Institutional Repository

An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide

An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide
An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide
A new acoustic-optical-microfluidic system is presented for the manipulation of bead-tagged DNA molecules. Acoustic radiation forces are used to manipulate microspheres into and away from the evanescent field of a laser coupled waveguide that is integrated into the reflector of the acoustic chamber. With suitable fluorophores the presence of the target DNA can be detected with a fluorescence microscope enabling large populations of beads to be examined simultaneously. The integrated waveguide and multimodal acoustic chamber are presented here, with results showing that the microspheres can be successfully detected as they are brought into the evanescent field using a quarter-wave acoustic configuration. It is also shown that by measuring the time of flight of a microsphere between the half- and quarterwave nodal planes the bead size can be determined, providing a means of multiplexing the detection (detecting a range of different target DNA sequences).
0001-4966
p.3925
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Townsend, Rosemary J.
f40068ec-6cf6-4896-827a-379b600b032b
Harris, Nicholas R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Zhang, Fan
22a0ac7a-2068-4eef-a441-c8efb8f24ed0
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Townsend, Rosemary J.
f40068ec-6cf6-4896-827a-379b600b032b
Harris, Nicholas R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Zhang, Fan
22a0ac7a-2068-4eef-a441-c8efb8f24ed0
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Glynne-Jones, Peter, Hill, Martyn, Townsend, Rosemary J., Harris, Nicholas R., Wilkinson, James S., Zhang, Fan and Melvin, Tracy (2008) An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide. Journal of the Acoustical Society of America, 123 (5), p.3925. (doi:10.1121/1.2935963).

Record type: Article

Abstract

A new acoustic-optical-microfluidic system is presented for the manipulation of bead-tagged DNA molecules. Acoustic radiation forces are used to manipulate microspheres into and away from the evanescent field of a laser coupled waveguide that is integrated into the reflector of the acoustic chamber. With suitable fluorophores the presence of the target DNA can be detected with a fluorescence microscope enabling large populations of beads to be examined simultaneously. The integrated waveguide and multimodal acoustic chamber are presented here, with results showing that the microspheres can be successfully detected as they are brought into the evanescent field using a quarter-wave acoustic configuration. It is also shown that by measuring the time of flight of a microsphere between the half- and quarterwave nodal planes the bead size can be determined, providing a means of multiplexing the detection (detecting a range of different target DNA sequences).

This record has no associated files available for download.

More information

Published date: May 2008
Additional Information: (Paper 5pPAa2 at Acoustics '08, 4 July, Paris )

Identifiers

Local EPrints ID: 63332
URI: http://eprints.soton.ac.uk/id/eprint/63332
ISSN: 0001-4966
PURE UUID: 2c4f8b75-19d9-4450-a461-b4495d23895d
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for Nicholas R. Harris: ORCID iD orcid.org/0000-0003-4122-2219
ORCID for James S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 08 Oct 2008
Last modified: 16 Mar 2024 03:10

Export record

Altmetrics

Contributors

Author: Martyn Hill ORCID iD
Author: Rosemary J. Townsend
Author: Nicholas R. Harris ORCID iD
Author: Fan Zhang
Author: Tracy Melvin

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×