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Discrimination and analysis of phytoplankton using a microfluidic cytometer

Discrimination and analysis of phytoplankton using a microfluidic cytometer
Discrimination and analysis of phytoplankton using a microfluidic cytometer
Identification and analysis of phytoplankton is important for understanding the environmental parameters that are influenced by the oceans, including pollution and climate change. Phytoplanktons are studied at the single cell level using conventional light-field and fluorescence microscopy, but the technique is labour intensive. Flow cytometry enables rapid and quantitative measurements of single cells and is now used as an analytical tool in phytoplankton analysis. However, it has a number of drawbacks, including high cost and portability. We describe the fabrication of a microfluidic (lab-on-a-chip) device for high-speed analysis of single phytoplankton. The device measures fluorescence (at three wavelength ranges) and the electrical impedance of single particles. The system was tested using a mixture of three algae (Isochrysis Galbana, Rhodosorus m., Synechococcus sp.) and the results compared with predictions from theory and measurements using a commercial flow cytometer (BD FACSAria). It is shown that the microfluidic flow cytometer is able to distinguish and characterise these different taxa and that impedance spectroscopy enables measurement of phytoplankton biophysical properties.
1751-8741
94-101
Benazzi, G.
0a1faa41-fbc8-426b-a942-f35d44757ebd
Holmes, D.
0bd269a5-3bab-477c-8bb0-a8711a0aa14b
Sun, T.
58b9d731-40fb-4f2f-b43a-77a5b889d03b
Mowlem, M.C.
6f633ca2-298f-48ee-a025-ce52dd62124f
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Benazzi, G.
0a1faa41-fbc8-426b-a942-f35d44757ebd
Holmes, D.
0bd269a5-3bab-477c-8bb0-a8711a0aa14b
Sun, T.
58b9d731-40fb-4f2f-b43a-77a5b889d03b
Mowlem, M.C.
6f633ca2-298f-48ee-a025-ce52dd62124f
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Benazzi, G., Holmes, D., Sun, T., Mowlem, M.C. and Morgan, H. (2007) Discrimination and analysis of phytoplankton using a microfluidic cytometer. IET Nanobiotechnology, 1 (6), 94-101. (doi:10.1049/iet-nbt:20070020).

Record type: Article

Abstract

Identification and analysis of phytoplankton is important for understanding the environmental parameters that are influenced by the oceans, including pollution and climate change. Phytoplanktons are studied at the single cell level using conventional light-field and fluorescence microscopy, but the technique is labour intensive. Flow cytometry enables rapid and quantitative measurements of single cells and is now used as an analytical tool in phytoplankton analysis. However, it has a number of drawbacks, including high cost and portability. We describe the fabrication of a microfluidic (lab-on-a-chip) device for high-speed analysis of single phytoplankton. The device measures fluorescence (at three wavelength ranges) and the electrical impedance of single particles. The system was tested using a mixture of three algae (Isochrysis Galbana, Rhodosorus m., Synechococcus sp.) and the results compared with predictions from theory and measurements using a commercial flow cytometer (BD FACSAria). It is shown that the microfluidic flow cytometer is able to distinguish and characterise these different taxa and that impedance spectroscopy enables measurement of phytoplankton biophysical properties.

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Published date: 2007

Identifiers

Local EPrints ID: 63914
URI: https://eprints.soton.ac.uk/id/eprint/63914
ISSN: 1751-8741
PURE UUID: d1de95b8-2b57-4a3d-8385-32d559ec976f
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

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Date deposited: 18 Nov 2008
Last modified: 20 Jul 2019 00:59

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