Isolation and characterization of microsatellite loci in the deep-sea marine fish, the roundnose grenadier (Coryphaenoides rupestris)

Knutsen, Halvor, Le Goff-Vitry, Marie, Fiani, David and Hoelzel, A. Rus (2008) Isolation and characterization of microsatellite loci in the deep-sea marine fish, the roundnose grenadier (Coryphaenoides rupestris). Molecular Ecology Resources, 8 (5), 993-995. (doi:10.1111/j.1755-0998.2008.02132.x).

Abstract

We developed polymerase chain reaction primers for eight dinucleotide microsatellite loci
in the marine deep sea fish, roundnose grenadier (Coryphaenoides rupestris). All markers
were obtained from a partial genomic DNA library, and characterized in 90 unrelated individuals
from one putative population sampled on the Mid-Atlantic Ridge. The number of
alleles ranged from two to 61 with an average of 21 per locus. The observed heterozygosity
levels ranged from 0.301 to 0.987 with an average of 0.672. Several of the markers amplified
multiple alleles from either the Atlantic cod (Gadus morhua) or the deep-sea fish roughhead
grenadier (Macrourus berglax).

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