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Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays

Record type: Article

In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5‘-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcelluar information about local Ca2+ flux.

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Citation

Yin, Huabing, Pattrick, Nicola, Zhang, Xunli, Klauke, Norbert, Cordingley, Hayley C., Haswell, Steven J. and Cooper, Jonathan M. (2008) Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays Analytical Chemistry, 80, (1), pp. 179-185. (doi:10.1021/ac701958z).

More information

e-pub ahead of print date: 6 December 2007
Published date: 1 January 2008

Identifiers

Local EPrints ID: 64826
URI: http://eprints.soton.ac.uk/id/eprint/64826
ISSN: 0003-2700
PURE UUID: 14725045-c042-4b7d-9d83-42d0e0d2055e

Catalogue record

Date deposited: 16 Jan 2009
Last modified: 17 Jul 2017 14:11

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Contributors

Author: Huabing Yin
Author: Nicola Pattrick
Author: Xunli Zhang
Author: Norbert Klauke
Author: Hayley C. Cordingley
Author: Steven J. Haswell
Author: Jonathan M. Cooper

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