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Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays

Yin, Huabing, Pattrick, Nicola, Zhang, Xunli, Klauke, Norbert, Cordingley, Hayley C., Haswell, Steven J. and Cooper, Jonathan M. (2008) Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays Analytical Chemistry, 80, (1), pp. 179-185. (doi:10.1021/ac701958z).

Record type: Article


In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5‘-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcelluar information about local Ca2+ flux.

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e-pub ahead of print date: 6 December 2007
Published date: 1 January 2008


Local EPrints ID: 64826
ISSN: 0003-2700
PURE UUID: 14725045-c042-4b7d-9d83-42d0e0d2055e

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Date deposited: 16 Jan 2009
Last modified: 17 Jul 2017 14:11

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Author: Huabing Yin
Author: Nicola Pattrick
Author: Xunli Zhang
Author: Norbert Klauke
Author: Hayley C. Cordingley
Author: Steven J. Haswell
Author: Jonathan M. Cooper

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