Attachment and detachment of living cells on modified microchannel surfaces in a microfluidic-based lab-on-a-chip system. [In IMRET9 Special Issue: Microreaction Technology IMRET 9: Proceedings of the Ninth International Conference on Microreaction Technology]
Attachment and detachment of living cells on modified microchannel surfaces in a microfluidic-based lab-on-a-chip system. [In IMRET9 Special Issue: Microreaction Technology IMRET 9: Proceedings of the Ninth International Conference on Microreaction Technology]
The attachment/detachment of living cells on modified microfluidic channel surfaces has been investigated using a “lab-on-a-chip” system. Cell attachment was mediated using surface modifications of the microchannel based on three different reagents, namely, 3-aminopropytriethoxysilane (APTES), glutaraldehyde and collagen, whilst the detachment was carried out by flowing media through the microchannel at increasing flow rates. All three surface modification methods showed significant improvement for Chinese hamster ovary (CHO) cells’ attachment, compared to that for an unmodified glass surface. The attachment/detachment of an additional four cell types, namely, T47D, U937, CaCo2 and NCTC 2544 cells, were also examined using the APTES modified channel surface. The observation of cell deformation suggested that the control of a shear stress within an optimal range enhances the cell adhesion to the surface. A theoretical model for fitting the measured detachment data is reported based on flow shear stress and the contribution from both surface adhesion bonds and hydrodynamic viscous stresses. It was demonstrated that the microfluidic system provided an easy and controllable approach to examine the attachment/detachment of a range of cells on different modified surfaces.
living cells, microfluidics, surface modification, shear stress, cell detachment
S82-S88
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Jones, Paul
b51472fd-cbaa-4297-97ee-fd0e47ea46e4
Haswell, Stephen J.
443a65de-9f13-4fbf-8b70-7de24004957b
15 January 2008
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Jones, Paul
b51472fd-cbaa-4297-97ee-fd0e47ea46e4
Haswell, Stephen J.
443a65de-9f13-4fbf-8b70-7de24004957b
Zhang, Xunli, Jones, Paul and Haswell, Stephen J.
(2008)
Attachment and detachment of living cells on modified microchannel surfaces in a microfluidic-based lab-on-a-chip system. [In IMRET9 Special Issue: Microreaction Technology IMRET 9: Proceedings of the Ninth International Conference on Microreaction Technology].
Chemical Engineering Journal, 135 (Supplement 1), .
(doi:10.1016/j.cej.2007.07.054).
Abstract
The attachment/detachment of living cells on modified microfluidic channel surfaces has been investigated using a “lab-on-a-chip” system. Cell attachment was mediated using surface modifications of the microchannel based on three different reagents, namely, 3-aminopropytriethoxysilane (APTES), glutaraldehyde and collagen, whilst the detachment was carried out by flowing media through the microchannel at increasing flow rates. All three surface modification methods showed significant improvement for Chinese hamster ovary (CHO) cells’ attachment, compared to that for an unmodified glass surface. The attachment/detachment of an additional four cell types, namely, T47D, U937, CaCo2 and NCTC 2544 cells, were also examined using the APTES modified channel surface. The observation of cell deformation suggested that the control of a shear stress within an optimal range enhances the cell adhesion to the surface. A theoretical model for fitting the measured detachment data is reported based on flow shear stress and the contribution from both surface adhesion bonds and hydrodynamic viscous stresses. It was demonstrated that the microfluidic system provided an easy and controllable approach to examine the attachment/detachment of a range of cells on different modified surfaces.
This record has no associated files available for download.
More information
Published date: 15 January 2008
Keywords:
living cells, microfluidics, surface modification, shear stress, cell detachment
Identifiers
Local EPrints ID: 64828
URI: http://eprints.soton.ac.uk/id/eprint/64828
ISSN: 1385-8947
PURE UUID: bb94cb40-e1f0-4b8b-93db-be5d49b1a80f
Catalogue record
Date deposited: 16 Jan 2009
Last modified: 16 Mar 2024 03:55
Export record
Altmetrics
Contributors
Author:
Paul Jones
Author:
Stephen J. Haswell
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
