A microfluidic based system for analysis of single cells based on Ca2+ flux (In special issue: Miniaturization)


Zhang, Xunli, Yin, Huabing, Cooper, Jon M. and Haswell, Stephen J. (2006) A microfluidic based system for analysis of single cells based on Ca2+ flux (In special issue: Miniaturization) Electrophoresis, 27, (24), pp. 5093-5100. (doi:10.1002/elps.200600390).

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Description/Abstract

A microfluidic format-based system has been developed for in situ monitoring of the calcium flux response to agonists using Chinese hamster ovary (CHO) cells. The assay is based on measuring the fluorescent intensity of the calcium-sensitive indicator, Fluo-4 AM, and was performed in a modified glass chip channel, whose surface was functionalised using a silanisation method with 3-aminopropyltriethoxysilane (APTS) (enabling the cells to be immobilised on the channel surface). CHO cells calcium flux response was measured for different agonists over a range of concentrations. Cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1002/elps.200600390
ISSNs: 0173-0835 (print)
Subjects:
ePrint ID: 64830
Date :
Date Event
November 2006Published
Date Deposited: 16 Jan 2009
Last Modified: 16 Apr 2017 17:18
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/64830

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