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mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures

Kredel, Simone, Oswald, Franz, Nienhaus, Karin, Deuschle, Karen, Röcke, Carlheinz, Wolff, Michael, Heilker, Ralf, Nienhaus, G. Ulrich and Wiedenmann, Jörg (2009) mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures PLoS ONE, 4, (2), e4391. (doi:10.1371/journal.pone.0004391).

Record type: Article

Abstract

A monomeric variant of the red fluorescent protein eqFP611, mRuby, is described. With excitation and emission maxima at 558 nm and 605 nm, respectively, and a large Stokes shift of 47 nm, mRuby appears particularly useful for imaging applications. The protein shows an exceptional resistance to denaturation at pH extremes. Moreover, mRuby is about ten-fold brighter compared to EGFP when being targeted to the endoplasmic reticulum. The engineering process of eqFP611 revealed that the C-terminal tail of the protein acts as a natural peroxisomal targeting signal (PTS). Using an mRuby variant carrying the eqFP611-PTS, we discovered that ordered inheritance of peroxisomes is widespread during mitosis of different mammalian cell types. The ordered partitioning is realized by the formation of peroxisome clusters around the poles of the mitotic spindle and ensures that equal numbers of the organelle are inherited by the daughter cells. The unique spectral properties make mRuby the marker of choice for a multitude of cell biological applications. Moreover, the use of mRuby has allowed novel insights in the biology of organelles responsible for severe human diseases.

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Published date: 2009

Identifiers

Local EPrints ID: 65392
URI: http://eprints.soton.ac.uk/id/eprint/65392
ISSN: 1932-6203
PURE UUID: 55a16a6f-919d-41de-9e1e-ce1fa62c8755
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

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Date deposited: 05 Feb 2009
Last modified: 19 Jul 2017 00:34

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Contributors

Author: Simone Kredel
Author: Franz Oswald
Author: Karin Nienhaus
Author: Karen Deuschle
Author: Carlheinz Röcke
Author: Michael Wolff
Author: Ralf Heilker
Author: G. Ulrich Nienhaus

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