Glynne-Jones, Peter, Boltryk, Rosemary J., Hill, Martyn, Zhang, Fan, Dong, Liqin, Wilkinson, James S., Melvin, Tracy, Harris, Nicholas R. and Brown, Tom (2009) Flexible acoustic particle manipulation device with integrated optical waveguide for enhanced microbead assays. Analytical Sciences, 25 (2), 285-291. (doi:10.2116/analsci.25.285).
Abstract
Realisation of a device intended for the manipulation and detection of bead-tagged DNA and other bio-molecules is presented. Acoustic radiation forces are used to manipulate polystyrene micro-beads into an optical evanescent field generated by a laser pumped ion-exchanged waveguide. The evanescent field only excites fluorophores brought within ~100 nm of the waveguide, allowing the system to differentiate between targets bound to the beads and those unbound and still held in suspension. The radiation forces are generated in a standing-wave chamber that supports multiple acoustic modes, permitting particles to be both attracted to the waveguide surface and also repelled. To provide further control over particle position, a novel method of switching rapidly between different acoustic modes is demonstrated, through which particles are manipulated into an arbitrary position within the chamber. A novel type of assay is presented: a mixture of streptavidin coated and control beads are driven towards a biotin functionalised surface, then a repulsive force is applied, making it possible to determine which beads became bound to the surface. It is shown that the quarter-wave mode can enhance bead to surface interaction, overcoming potential barriers caused by surface charges. It is demonstrated that by measuring the time of flight of a microsphere across the device the bead size can be determined, providing a means of multiplexing the detection, potentially detecting a range of different target molecules, or varying bead mass.
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- Faculties (pre 2011 reorg) > Faculty of Engineering Science & Maths (pre 2011 reorg) > Optoelectronics Research Centre (pre 2011 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > Zepler Institute for Photonics and Nanoelectronics > Optoelectronics Research Centre (pre 2011 reorg)
Zepler Institute for Photonics and Nanoelectronics > Optoelectronics Research Centre (pre 2011 reorg) - Current Faculties > Faculty of Engineering and Physical Sciences > School of Engineering > Mechanical Engineering > Mechatronics
Mechanical Engineering > Mechatronics - Faculties (pre 2011 reorg) > Faculty of Engineering Science & Maths (pre 2011 reorg) > Chemistry (pre 2011 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > School of Chemistry > Chemistry (pre 2011 reorg)
School of Chemistry > Chemistry (pre 2011 reorg) - Faculties (pre 2011 reorg) > Faculty of Engineering Science & Maths (pre 2011 reorg) > Electronics & Computer Science (pre 2011 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > School of Electronics and Computer Science > Electronics & Computer Science (pre 2011 reorg)
School of Electronics and Computer Science > Electronics & Computer Science (pre 2011 reorg) - Current Faculties > Faculty of Engineering and Physical Sciences > School of Electronics and Computer Science > Smart Electronic Materials and Systems
School of Electronics and Computer Science > Smart Electronic Materials and Systems - Faculties (pre 2011 reorg) > Faculty of Engineering Science & Maths (pre 2011 reorg) > Engineering Sciences (pre 2011 reorg) > Electro-Mechanical Engineering (pre 2011 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > School of Engineering > Mechanical Engineering > Electro-Mechanical Engineering (pre 2011 reorg)
Mechanical Engineering > Electro-Mechanical Engineering (pre 2011 reorg)
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