The University of Southampton
University of Southampton Institutional Repository

Flexible acoustic particle manipulation device with integrated optical waveguide for enhanced microbead assays

Flexible acoustic particle manipulation device with integrated optical waveguide for enhanced microbead assays
Flexible acoustic particle manipulation device with integrated optical waveguide for enhanced microbead assays
Realisation of a device intended for the manipulation and detection of bead-tagged DNA and other bio-molecules is presented. Acoustic radiation forces are used to manipulate polystyrene micro-beads into an optical evanescent field generated by a laser pumped ion-exchanged waveguide. The evanescent field only excites fluorophores brought within ~100 nm of the waveguide, allowing the system to differentiate between targets bound to the beads and those unbound and still held in suspension. The radiation forces are generated in a standing-wave chamber that supports multiple acoustic modes, permitting particles to be both attracted to the waveguide surface and also repelled. To provide further control over particle position, a novel method of switching rapidly between different acoustic modes is demonstrated, through which particles are manipulated into an arbitrary position within the chamber. A novel type of assay is presented: a mixture of streptavidin coated and control beads are driven towards a biotin functionalised surface, then a repulsive force is applied, making it possible to determine which beads became bound to the surface. It is shown that the quarter-wave mode can enhance bead to surface interaction, overcoming potential barriers caused by surface charges. It is demonstrated that by measuring the time of flight of a microsphere across the device the bead size can be determined, providing a means of multiplexing the detection, potentially detecting a range of different target molecules, or varying bead mass.
0910-6340
285-291
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Boltryk, Rosemary J.
0452b21c-a758-4d4a-925b-1511d9296d62
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Zhang, Fan
22a0ac7a-2068-4eef-a441-c8efb8f24ed0
Dong, Liqin
7d24399e-c2f7-42e7-a33a-3389cba6c1fa
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Harris, Nicholas R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Boltryk, Rosemary J.
0452b21c-a758-4d4a-925b-1511d9296d62
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Zhang, Fan
22a0ac7a-2068-4eef-a441-c8efb8f24ed0
Dong, Liqin
7d24399e-c2f7-42e7-a33a-3389cba6c1fa
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Harris, Nicholas R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Glynne-Jones, Peter, Boltryk, Rosemary J., Hill, Martyn, Zhang, Fan, Dong, Liqin, Wilkinson, James S., Melvin, Tracy, Harris, Nicholas R. and Brown, Tom (2009) Flexible acoustic particle manipulation device with integrated optical waveguide for enhanced microbead assays Analytical Sciences, 25, (2), pp. 285-291.

Record type: Article

Abstract

Realisation of a device intended for the manipulation and detection of bead-tagged DNA and other bio-molecules is presented. Acoustic radiation forces are used to manipulate polystyrene micro-beads into an optical evanescent field generated by a laser pumped ion-exchanged waveguide. The evanescent field only excites fluorophores brought within ~100 nm of the waveguide, allowing the system to differentiate between targets bound to the beads and those unbound and still held in suspension. The radiation forces are generated in a standing-wave chamber that supports multiple acoustic modes, permitting particles to be both attracted to the waveguide surface and also repelled. To provide further control over particle position, a novel method of switching rapidly between different acoustic modes is demonstrated, through which particles are manipulated into an arbitrary position within the chamber. A novel type of assay is presented: a mixture of streptavidin coated and control beads are driven towards a biotin functionalised surface, then a repulsive force is applied, making it possible to determine which beads became bound to the surface. It is shown that the quarter-wave mode can enhance bead to surface interaction, overcoming potential barriers caused by surface charges. It is demonstrated that by measuring the time of flight of a microsphere across the device the bead size can be determined, providing a means of multiplexing the detection, potentially detecting a range of different target molecules, or varying bead mass.

PDF 65708-01.pdf - Version of Record
Download (856kB)

More information

Published date: 10 February 2009
Organisations: Chemistry, Optoelectronics Research Centre, Electro-Mechanical Engineering, Electronics & Computer Science

Identifiers

Local EPrints ID: 65708
URI: http://eprints.soton.ac.uk/id/eprint/65708
ISSN: 0910-6340
PURE UUID: 7ee4f5ba-9422-4f74-a285-8720f22bbcf4
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for James S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697
ORCID for Nicholas R. Harris: ORCID iD orcid.org/0000-0003-4122-2219

Catalogue record

Date deposited: 16 Mar 2009
Last modified: 28 Oct 2017 18:08

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×