The University of Southampton
University of Southampton Institutional Repository

E-Cadherin regulates neural stem cell self-renewal

Karpowicz, P., Willaime-Morawek, S., Balenci, L., DeVeale, B., Inoue, T. and van der Kooy, D. (2009) E-Cadherin regulates neural stem cell self-renewal Journal of Neuroscience, 29, (12), pp. 3885-3896. (doi:10.1523/JNEUROSCI.0037-09.2009).

Record type: Article

Abstract

E-Cadherin, a cell adhesion protein, has been shown to take part in the compartmentalization, proliferation, survival, and differentiation of cells. E-Cadherin is expressed in the adult and embryonic forebrain germinal zones in vivo, and in clonal colonies of cells derived from these regions and grown in vitro. Mice carrying E-Cadherin floxed genes crossed to mice expressing Cre under the Nestin promoter demonstrate defects in the self-renewal of neural stem cells both in vivo and in vitro. The functional role of E-Cadherin is further demonstrated using adhesion-blocking antibodies in vitro, which specifically target cadherin extracellular adhesive domains. Adult neural stem cell colonies decrease in the presence of E-Cadherin antibodies in a dosage-dependent manner, in contrast to P-Cadherin antibody. On overexpression of normal E-Cadherin and a mutated E-Cadherin, containing no intracellular binding domain, an increased number of clonal adult neural stem cell colonies are observed. These data suggest it is specifically E-Cadherin adhesion that is responsible for these self-renewal effects. These data show the importance of E-Cadherin in the neural stem cell niche and suggest E-Cadherin regulates the number of these cells.

PDF Karpowicz_JN_2009.pdf - Version of Record
Download (607kB)

More information

Published date: 25 March 2009
Organisations: Clinical Neurosciences

Identifiers

Local EPrints ID: 66445
URI: http://eprints.soton.ac.uk/id/eprint/66445
ISSN: 0270-6474
PURE UUID: ed50a39a-dbbf-4c13-9a83-492246e39794
ORCID for S. Willaime-Morawek: ORCID iD orcid.org/0000-0002-1121-6419

Catalogue record

Date deposited: 15 Jun 2009
Last modified: 19 Jul 2017 00:24

Export record

Altmetrics

Contributors

Author: P. Karpowicz
Author: L. Balenci
Author: B. DeVeale
Author: T. Inoue
Author: D. van der Kooy

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×