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Nitric oxide is a factor in the stabilization of hypoxia-inducible factor-1? in cancer: role of free radical formation

Nitric oxide is a factor in the stabilization of hypoxia-inducible factor-1? in cancer: role of free radical formation
Nitric oxide is a factor in the stabilization of hypoxia-inducible factor-1? in cancer: role of free radical formation
Widespread expression of the ?-subunit of hypoxia-induciblefactor (HIF-1?) was observed in samples of human oral squamous cell carcinoma. In all the cases, this was accompanied by a widespread distribution of nitric oxide (NO) synthases (NOS). Furthermore, in three human cell lines derived from human oral squamous cell carcinoma, the accumulation of HIF-1? was prevented either by inhibition of NOS activity with the nonspecific NOS inhibitor NG- monomethyl-L-arginine or by the antioxidants N-acetyl-Lcysteine and ascorbic acid. We suggest that, in certain forms of cancer, NO might be responsible for the accumulation of HIF-1? by a mechanism dependent on free radicals.
0008-5472
770-774
Quintero, Marisol
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Brennan, Peter A.
0d39fb85-652b-439f-9f8e-af61ec8a54f1
Thomas, Gareth J.
2ff54aa9-a766-416b-91ee-cf1c5be74106
Moncada, Salvador
0f661842-cd67-46e6-b82d-47f2c5e0e750
Quintero, Marisol
f6d85082-c9da-4fc9-8b6a-226216d429ff
Brennan, Peter A.
0d39fb85-652b-439f-9f8e-af61ec8a54f1
Thomas, Gareth J.
2ff54aa9-a766-416b-91ee-cf1c5be74106
Moncada, Salvador
0f661842-cd67-46e6-b82d-47f2c5e0e750

Quintero, Marisol, Brennan, Peter A., Thomas, Gareth J. and Moncada, Salvador (2006) Nitric oxide is a factor in the stabilization of hypoxia-inducible factor-1? in cancer: role of free radical formation. Cancer Research, 66 (2), 770-774. (doi:10.1158/0008-5472.CAN-05-0333).

Record type: Article

Abstract

Widespread expression of the ?-subunit of hypoxia-induciblefactor (HIF-1?) was observed in samples of human oral squamous cell carcinoma. In all the cases, this was accompanied by a widespread distribution of nitric oxide (NO) synthases (NOS). Furthermore, in three human cell lines derived from human oral squamous cell carcinoma, the accumulation of HIF-1? was prevented either by inhibition of NOS activity with the nonspecific NOS inhibitor NG- monomethyl-L-arginine or by the antioxidants N-acetyl-Lcysteine and ascorbic acid. We suggest that, in certain forms of cancer, NO might be responsible for the accumulation of HIF-1? by a mechanism dependent on free radicals.

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Published date: January 2006

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Local EPrints ID: 66688
URI: http://eprints.soton.ac.uk/id/eprint/66688
ISSN: 0008-5472
PURE UUID: 1fa5196f-1fc5-4077-b738-be9dcae09a0a

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Date deposited: 10 Jul 2009
Last modified: 13 Mar 2024 18:30

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Contributors

Author: Marisol Quintero
Author: Peter A. Brennan
Author: Salvador Moncada

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