The University of Southampton
University of Southampton Institutional Repository

Artificial oocyte maturation in Patella depressa and Patella vulgata using NaOH-alkalinized seawater

Artificial oocyte maturation in Patella depressa and Patella vulgata using NaOH-alkalinized seawater
Artificial oocyte maturation in Patella depressa and Patella vulgata using NaOH-alkalinized seawater
Laboratory investigations of environmental factors controlling early development and larval survival of free-spawning marine invertebrates are often limited by the difficulty of inducing spawning under laboratory conditions. Patellid gastropod species must be dissected in order to obtain the gametes from the gonads, often yielding unfertilizable immature eggs. The artificial maturation of oocytes through NaOH-alkalinization methods was investigated in order to improve laboratory experiments on larval ecology of Patella depressa and Patella vulgata. Maturation was accelerated above pH 9.0. Alkalinizing oocytes at pH 9.5 significantly enhanced in vitro fertilization rates, especially at higher sperm concentrations. pH 10 was harmful to eggs. Sperm dilution experiments using alkalinized oocytes suggest the use of sperm concentrations proximate to 106 spermatozoa ml-.
Fertilization, larval rearing, mollusc, oocyte maturation, Patella, pH
1745-1000
503-510
Aquino De Souza, Rosana
f951c03c-7de3-454d-bc27-e367ca0efc51
Tyler, Paul
d1965388-38cc-4c1d-9217-d59dba4dd7f8
Hawkins, Stephen John
758fe1c1-30cd-4ed1-bb65-2471dc7c11fa
Aquino De Souza, Rosana
f951c03c-7de3-454d-bc27-e367ca0efc51
Tyler, Paul
d1965388-38cc-4c1d-9217-d59dba4dd7f8
Hawkins, Stephen John
758fe1c1-30cd-4ed1-bb65-2471dc7c11fa

Aquino De Souza, Rosana, Tyler, Paul and Hawkins, Stephen John (2009) Artificial oocyte maturation in Patella depressa and Patella vulgata using NaOH-alkalinized seawater. Marine Biology Research, 5 (5), 503-510. (doi:10.1080/17451000802603645).

Record type: Article

Abstract

Laboratory investigations of environmental factors controlling early development and larval survival of free-spawning marine invertebrates are often limited by the difficulty of inducing spawning under laboratory conditions. Patellid gastropod species must be dissected in order to obtain the gametes from the gonads, often yielding unfertilizable immature eggs. The artificial maturation of oocytes through NaOH-alkalinization methods was investigated in order to improve laboratory experiments on larval ecology of Patella depressa and Patella vulgata. Maturation was accelerated above pH 9.0. Alkalinizing oocytes at pH 9.5 significantly enhanced in vitro fertilization rates, especially at higher sperm concentrations. pH 10 was harmful to eggs. Sperm dilution experiments using alkalinized oocytes suggest the use of sperm concentrations proximate to 106 spermatozoa ml-.

Text
DeSousa_etal_2009_MarBioRes.pdf - Version of Record
Restricted to Repository staff only
Request a copy

More information

Published date: September 2009
Keywords: Fertilization, larval rearing, mollusc, oocyte maturation, Patella, pH

Identifiers

Local EPrints ID: 68741
URI: https://eprints.soton.ac.uk/id/eprint/68741
ISSN: 1745-1000
PURE UUID: 5f593b35-9211-464c-a589-56d3d77e2f02

Catalogue record

Date deposited: 22 Sep 2009
Last modified: 19 Jul 2019 23:48

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×