The University of Southampton
University of Southampton Institutional Repository

DeltaNp73 regulates neuronal survival in vivo

Tissir, Fadel, Ravni, Aurélia, Achouri, Younès, Riethmacher, Dieter, Meyer, Gundela and Goffinet, Andre M. (2009) DeltaNp73 regulates neuronal survival in vivo Proceedings of the National Academy of Sciences, 106, (39), pp. 16871-16876. (doi:10.1073/pnas.0903191106).

Record type: Article

Abstract

Apoptosis occurs widely during brain development, and p73 transcription factors are thought to play essential roles in this process. The p73 transcription factors are present in two forms, the full length TAp73 and the N-terminally truncated DeltaNp73. In cultured sympathetic neurons, overexpression of DeltaNp73 inhibits apoptosis induced by nerve growth factor withdrawal or p53 overexpression. To probe the function of DeltaNp73 in vivo, we generated a null allele and inserted sequences encoding the recombinase Cre and green fluorescent protein (EGFP). We show that DeltaNp73 is heavily expressed in the thalamic eminence (TE) that contributes neurons to ventral forebrain, in vomeronasal neurons, Cajal-Retzius cells (CRc), and choroid plexuses. In DeltaNp73?/? mice, cells in preoptic areas, vomeronasal neurons, GnRH-positive cells, and CRc were severely reduced in number, and choroid plexuses were atrophic. This phenotype was enhanced when DeltaNp73–positive cells were ablated by diphtheria toxin expression. However, ablation of cells that express DeltaNp73 and Wnt3a did neither remove all CRc, nor did they abolish Reelin secretion or generate a reeler-like cortical phenotype. Our data emphasize the role of DeltaNp73 in neuronal survival in vivo and in choroid plexus development, the importance of the TE as a source of neurons in ventral forebrain, and the multiple origins of CRc, with redundant production of Reelin.

Full text not available from this repository.

More information

Published date: 29 September 2009
Keywords: Cajal-Retzius cells, p73, Reelin, thalamic eminence, vomeronasal nerve
Organisations: Human Genetics

Identifiers

Local EPrints ID: 68906
URI: http://eprints.soton.ac.uk/id/eprint/68906
ISSN: 0027-8424
PURE UUID: d0f58483-c2ff-4826-80bd-f3473cd64b2f

Catalogue record

Date deposited: 08 Oct 2009
Last modified: 19 Jul 2017 00:15

Export record

Altmetrics

Contributors

Author: Fadel Tissir
Author: Aurélia Ravni
Author: Younès Achouri
Author: Gundela Meyer
Author: Andre M. Goffinet

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×