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The subtle effects of sea water acidification on the amphipod Gammarus locusta

Hauton, C., Tyrrell, T. and Williams, J. (2009) The subtle effects of sea water acidification on the amphipod Gammarus locusta Biogeosciences, 6, (8), pp. 1479-1489. (doi:10.5194/bg-6-1479-2009).

Record type: Article

Abstract

We report an investigation of the effects of increases in pCO2 on the survival, growth and molecular physiology of the neritic amphipod Gammarus locusta which has a cosmopolitan distribution in estuaries. Amphipods were reared from juvenile to mature adult in laboratory microcosms at three different levels of pH in nominal range 8.1–7.6. Growth rate was estimated from weekly measures of body length. At sexual maturity the amphipods were sacrificed and assayed for changes in the expression of genes coding for a heat shock protein (hsp70 gene) and the metabolic enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh gene). The data show that the growth and survival of this species is not significantly impacted by a decrease in sea water pH of up to 0.5 units. Quantitative real-time PCR analysis indicated that there was no significant effect of growth in acidified sea water on the sustained expression of the hsp70 gene. There was a consistent and significant increase in the expression of the gapdh gene at a pH of ~7.5 which, when combined with observations from other workers, suggests that metabolic changes may occur in response to acidification. It is concluded that sensitive assays of tissue physiology and molecular biology should be routinely employed in future studies of the impacts of sea water acidification as subtle effects on the physiology and metabolism of coastal marine species may be overlooked in conventional gross "end-point" studies of organism growth or mortality.

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Published date: 2009
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 69149
URI: http://eprints.soton.ac.uk/id/eprint/69149
ISSN: 1726-4170
PURE UUID: dda658df-ad6a-4d75-9d28-4bb7d3f2acb8
ORCID for C. Hauton: ORCID iD orcid.org/0000-0002-2313-4226
ORCID for T. Tyrrell: ORCID iD orcid.org/0000-0002-1002-1716

Catalogue record

Date deposited: 20 Oct 2009
Last modified: 19 Jul 2017 00:13

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Contributors

Author: C. Hauton ORCID iD
Author: T. Tyrrell ORCID iD
Author: J. Williams

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