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Multi-modal particle manipulator to enhance bead-based bioassays

Glynne-Jones, P., Boltryk, R.J., Hill, M., Zhang, F., Dong, L., Wilkinson, J.S., Brown, T., Melvin, T. and Harris, N.R. (2010) Multi-modal particle manipulator to enhance bead-based bioassays Ultrasonics, 50, (2), pp. 235-239. (doi:10.1016/j.ultras.2009.09.025).

Record type: Article

Abstract

By sequentially pushing micro-beads towards and away from a sensing surface, we show that ultrasonic radiation forces can be used to enhance the interaction between a functionalised glass surface and polystyrene micro-beads, and identify those that bind to the surface by illuminating bound beads using an evanescent field generated by guided light. The movement towards and immobilisation of streptavidin coated beads onto a biotin functionalised waveguide surface is achieved by using a quarter-wavelength mode pushing beads onto the surface, while the removal of non-specifically bound beads uses a second quarter-wavelength mode which exhibits a kinetic energy maximum at the boundary between the carrier layer and fluid, drawing beads towards this surface. This has been achieved using a multi-modal acoustic device which exhibits both of these quarter-wavelength resonances. Both 1-D acoustic modelling and finite element analysis has been used to design this device and to investigate the spatial uniformity of the field. We demonstrate experimentally that 90% of specifically bound beads remain attached after applying ultrasound, with 80% of non-specifically bound control beads being successfully removed acoustically. This approach overcomes problems associated with lengthy sedimentation processes used for bead-based bioassays and surface (electrostatic) forces, which delay or prevent immobilisation. We explain the potential of this technique in the development of DNA and protein assays in terms of detection speed and multiplexing

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More information

Published date: February 2010
Keywords: acoustic radiation forces, bio-sensor, frequency switching, micro-beads, optical waveguide
Organisations: Optoelectronics Research Centre, Electro-Mechanical Engineering, Electronics & Computer Science

Identifiers

Local EPrints ID: 69843
URI: http://eprints.soton.ac.uk/id/eprint/69843
ISSN: 0041-624X
PURE UUID: f4ec45f4-77fc-404f-8a54-5cf062f8d46b
ORCID for P. Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for M. Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697
ORCID for N.R. Harris: ORCID iD orcid.org/0000-0003-4122-2219

Catalogue record

Date deposited: 08 Dec 2009
Last modified: 19 Jul 2017 00:05

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Contributors

Author: P. Glynne-Jones ORCID iD
Author: R.J. Boltryk
Author: M. Hill ORCID iD
Author: F. Zhang
Author: L. Dong
Author: J.S. Wilkinson ORCID iD
Author: T. Brown
Author: T. Melvin
Author: N.R. Harris ORCID iD

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