Short peptides derived from the BAG-1 C-terminus inhibit the interaction between BAG-1 and HSC70 and decrease breast cancer cell growth
Short peptides derived from the BAG-1 C-terminus inhibit the interaction between BAG-1 and HSC70 and decrease breast cancer cell growth
BAG-1, a multifunctional protein, interacts with a plethora of cellular targets where the interaction with HSC70 and HSP70, is considered vital. Structural studies have demonstrated the C-terminal of BAG-1 forms a bundle of three alpha-helices of which helices 2 and 3 are directly involved in binding to the chaperones. Here we found peptides derived from helices 2 and 3 of BAG-1 interfered with BAG-1:HSC70 binding. We confirmed that a 12 amino-acid peptide from helix 2 directly interacted with HSC70 and when introduced into MCF-7 and ZR-75-1 cells, these peptides inhibited their growth. In conclusion, we have identified a small domain within BAG-1 which appears to play a critical role in the interaction with HSC70
3405-3411
Sharp, Adam
f26a1894-b10f-4864-a314-750628d6c0cc
Cutress, Ramsey I.
68ae4f86-e8cf-411f-a335-cdba51797406
Johnson, Peter W.M.
3f6068ce-171e-4c2c-aca9-dc9b6a37413f
Packham, Graham
fdabe56f-2c58-469c-aadf-38878f233394
Townsend, Paul A.
a2680443-664e-46d0-b4dd-97456ba810db
3 November 2009
Sharp, Adam
f26a1894-b10f-4864-a314-750628d6c0cc
Cutress, Ramsey I.
68ae4f86-e8cf-411f-a335-cdba51797406
Johnson, Peter W.M.
3f6068ce-171e-4c2c-aca9-dc9b6a37413f
Packham, Graham
fdabe56f-2c58-469c-aadf-38878f233394
Townsend, Paul A.
a2680443-664e-46d0-b4dd-97456ba810db
Sharp, Adam, Cutress, Ramsey I., Johnson, Peter W.M., Packham, Graham and Townsend, Paul A.
(2009)
Short peptides derived from the BAG-1 C-terminus inhibit the interaction between BAG-1 and HSC70 and decrease breast cancer cell growth.
FEBS Letters, 583 (21), .
(doi:10.1016/j.febslet.2009.09.047).
Abstract
BAG-1, a multifunctional protein, interacts with a plethora of cellular targets where the interaction with HSC70 and HSP70, is considered vital. Structural studies have demonstrated the C-terminal of BAG-1 forms a bundle of three alpha-helices of which helices 2 and 3 are directly involved in binding to the chaperones. Here we found peptides derived from helices 2 and 3 of BAG-1 interfered with BAG-1:HSC70 binding. We confirmed that a 12 amino-acid peptide from helix 2 directly interacted with HSC70 and when introduced into MCF-7 and ZR-75-1 cells, these peptides inhibited their growth. In conclusion, we have identified a small domain within BAG-1 which appears to play a critical role in the interaction with HSC70
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Published date: 3 November 2009
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Edited by Lukas Huber
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Local EPrints ID: 69869
URI: http://eprints.soton.ac.uk/id/eprint/69869
ISSN: 0014-5793
PURE UUID: 4bd6c940-bebb-40fe-a3ba-afd1e2bafb02
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Date deposited: 09 Dec 2009
Last modified: 14 Mar 2024 02:44
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Author:
Adam Sharp
Author:
Paul A. Townsend
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