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Interaction with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage

Interaction with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage
Interaction with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage
Polymeric medical devices widely used in orthopedic surgery play key roles in fracture fixation and orthopedic implant design. Topographical modification and surface micro-roughness of these devices regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have evolved the field of surface modification; in particular, nanotechnology has allowed the development of nanoscale substrates for the investigation into cell-nanofeature interactions. In this study human osteoblasts (HOBs) were cultured on ordered nanoscale pits and random nano craters and islands. Adhesion subtypes were quantified by immunofluorescent microscopy and cell-substrate interactions investigated via immuno-scanning electron microscopy. To investigate the effects of these substrates on cellular function 1.7 k microarray analysis was used to establish gene profiles of enriched STRO-1+ progenitor cell populations cultured on these nanotopographies. Nanotopographies affected the formation of adhesions on experimental substrates. Adhesion formation was prominent on planar control substrates and reduced on nanocrater and nanoisland topographies; nanopits, however, were shown to inhibit directly the formation of large adhesions. STRO-1+ progenitor cells cultured on experimental substrates revealed significant changes in genetic expression. This study implicates nanotopographical modification as a significant modulator of osteoblast adhesion and cellular function in mesenchymal populations.
osteoblasts, mesenchymal stem cells, focal adhesions, genomic regulation, nanotopography, nanobioscience
1549-3296
195-208
Biggs, Manus J.
90caf50f-5da2-437b-a957-d9cc57bebb14
Richards, R. Geoff
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Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
McMurray, Rebecca J.
2e4cf997-4e4a-4daf-9d49-09b83abde8cf
Affrossman, Stanley
bf16b996-406e-47cd-b907-815fdea21201
Wilkinson, Chris D.W.
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Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Mathew J.
01b213f4-fafa-4131-9b36-003fcfbd004e
Biggs, Manus J.
90caf50f-5da2-437b-a957-d9cc57bebb14
Richards, R. Geoff
69d42d1f-d1dd-4f1b-8766-03df55a5f2ca
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
McMurray, Rebecca J.
2e4cf997-4e4a-4daf-9d49-09b83abde8cf
Affrossman, Stanley
bf16b996-406e-47cd-b907-815fdea21201
Wilkinson, Chris D.W.
ea556659-2ba1-43a8-b8d3-37e4bfaffd41
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Mathew J.
01b213f4-fafa-4131-9b36-003fcfbd004e

Biggs, Manus J., Richards, R. Geoff, Gadegaard, Nikolaj, McMurray, Rebecca J., Affrossman, Stanley, Wilkinson, Chris D.W., Oreffo, Richard O.C. and Dalby, Mathew J. (2008) Interaction with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. Journal of Biomedical Materials Research Part A, 91A (1), 195-208. (doi:10.1002/jbm.a.32196).

Record type: Article

Abstract

Polymeric medical devices widely used in orthopedic surgery play key roles in fracture fixation and orthopedic implant design. Topographical modification and surface micro-roughness of these devices regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have evolved the field of surface modification; in particular, nanotechnology has allowed the development of nanoscale substrates for the investigation into cell-nanofeature interactions. In this study human osteoblasts (HOBs) were cultured on ordered nanoscale pits and random nano craters and islands. Adhesion subtypes were quantified by immunofluorescent microscopy and cell-substrate interactions investigated via immuno-scanning electron microscopy. To investigate the effects of these substrates on cellular function 1.7 k microarray analysis was used to establish gene profiles of enriched STRO-1+ progenitor cell populations cultured on these nanotopographies. Nanotopographies affected the formation of adhesions on experimental substrates. Adhesion formation was prominent on planar control substrates and reduced on nanocrater and nanoisland topographies; nanopits, however, were shown to inhibit directly the formation of large adhesions. STRO-1+ progenitor cells cultured on experimental substrates revealed significant changes in genetic expression. This study implicates nanotopographical modification as a significant modulator of osteoblast adhesion and cellular function in mesenchymal populations.

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More information

Published date: 23 September 2008
Keywords: osteoblasts, mesenchymal stem cells, focal adhesions, genomic regulation, nanotopography, nanobioscience

Identifiers

Local EPrints ID: 70293
URI: https://eprints.soton.ac.uk/id/eprint/70293
ISSN: 1549-3296
PURE UUID: ece68746-65e0-4876-b795-0babc967d4db
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 08 Mar 2010
Last modified: 01 Oct 2019 00:55

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Contributors

Author: Manus J. Biggs
Author: R. Geoff Richards
Author: Nikolaj Gadegaard
Author: Rebecca J. McMurray
Author: Stanley Affrossman
Author: Chris D.W. Wilkinson
Author: Mathew J. Dalby

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