Proteomic analysis of human osteoprogenitor response to disordered nanotopography

Kantawong, Fahsai, Burchmore, Richard, Gadegaard, Nikolaj, Oreffo, Richard O.C. and Dalby, Matthew J. (2008) Proteomic analysis of human osteoprogenitor response to disordered nanotopography. Journal of the Royal Society Interface, 6 (40), 1075-1086. (doi:10.1098/?rsif.2008.0447).

Abstract

Previous studies have shown that microgroove-initiated contact guidance can induce bone formation in osteoprogenitor cells (OPGs) and produce changes in the cell proteome. For proteomic analysis, differential in-gel electrophoresis (DIGE) can be used as a powerful diagnostic method to provide comparable data between the proteomic profiles of cells cultured in different conditions. This study focuses on the response of OPGs to a novel nanoscale pit topography with osteoinductive properties compared with planar controls. Disordered near-square nanopits with 120nm diameter and 100nm depth with an average 300nm centre-to-centre spacing (300nm spaced pits in square pattern, but with +/-50nm disorder) were fabricated on 1x1cm2 polycaprolactone sheets. Human OPGs were seeded onto the test materials. DIGE analysis revealed changes in the expression of a number of distinct proteins, including upregulation of actin isoforms, beta-galectin1, vimentin and procollagen-proline, 2-oxoglutarate 4-dioxygenase and prolyl 4-hydroxylase. Downregulation of enolase, caldesmon, zyxin, GRASP55, Hsp70 (BiP/GRP78), RNH1, cathepsin D and Hsp27 was also observed. The differences in cell morphology and mineralization are also reported using histochemical techniques

This record has no associated files available for download.

Contributors

Author: Richard O.C. Oreffo

Download statistics