Translation termination reinitiation between open reading frame 1 (ORF1) and ORF2 enables capsid expression in a bovine norovirus without the need for production of viral subgenomic RNA
Translation termination reinitiation between open reading frame 1 (ORF1) and ORF2 enables capsid expression in a bovine norovirus without the need for production of viral subgenomic RNA
A generally accepted view of norovirus replication is that capsid expression requires production of a subgenomic transcript, the presence of capsid often being used as a surrogate marker to indicate the occurrence of viral replication. Using a polymerase II-based baculovirus delivery system, we observed capsid expression following introduction of a full-length genogroup 3 norovirus genome into HepG2 cells. However, capsid expression occurred as a result of a novel translation termination/reinitiation event between the nonstructural-protein and capsid open reading frames, a feature that may be unique to genogroup 3 noroviruses.
8917-8921
McCormick, Christopher J.
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Salim, Omar
a8a00604-9e57-4638-8c96-5b37e75fee90
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
September 2008
McCormick, Christopher J.
0fce14bf-2f67-4d08-991f-114dd1e7f0bd
Salim, Omar
a8a00604-9e57-4638-8c96-5b37e75fee90
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCormick, Christopher J., Salim, Omar, Lambden, Paul R. and Clarke, Ian N.
(2008)
Translation termination reinitiation between open reading frame 1 (ORF1) and ORF2 enables capsid expression in a bovine norovirus without the need for production of viral subgenomic RNA.
Journal of Virology, 82 (17), .
(doi:10.1128/JVI.02362-07).
(PMID:18579601)
Abstract
A generally accepted view of norovirus replication is that capsid expression requires production of a subgenomic transcript, the presence of capsid often being used as a surrogate marker to indicate the occurrence of viral replication. Using a polymerase II-based baculovirus delivery system, we observed capsid expression following introduction of a full-length genogroup 3 norovirus genome into HepG2 cells. However, capsid expression occurred as a result of a novel translation termination/reinitiation event between the nonstructural-protein and capsid open reading frames, a feature that may be unique to genogroup 3 noroviruses.
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e-pub ahead of print date: 25 June 2008
Published date: September 2008
Organisations:
Medicine
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Local EPrints ID: 70850
URI: http://eprints.soton.ac.uk/id/eprint/70850
ISSN: 0022-538X
PURE UUID: 22d699f3-069f-4409-821f-c10e5b6d0dbc
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Date deposited: 09 Feb 2010
Last modified: 14 Mar 2024 02:50
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Author:
Omar Salim
Author:
Paul R. Lambden
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