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The role of eosinophil major basic protein in angiogenesis

The role of eosinophil major basic protein in angiogenesis
The role of eosinophil major basic protein in angiogenesis
BACKGROUND: Eosinophil-derived major basic protein (MBP) plays an active role in allergic inflammation and tissue remodelling. However, its role in angiogenesis has not been established as yet. Therefore our objective was to investigate whether MBP exhibits any direct pro-angiogenic effects. METHODS: Rat aortic endothelial cells and human umbilical vascular endothelial cells were cultured with different concentrations of MBP and their viability (Trypan blue exclusion test), proliferation (thymidine incorporation) and capillary-like structure formation (matrigel assay) were investigated in vitro. The angiogenic activity of MBP was then tested in vivo using the chick chorio allantoic membrane (CAM) assay. RESULTS: Subcytotoxic concentrations of MBP induce endothelial cell proliferation and enhance the pro-mitogenic effect of vascular endothelial growth factor (VEGF), but do not affect their VEGF release. MBP promotes capillarogenesis by endothelial cells seeded on matrigel and sprouting formation in the CAM assay. Furthermore, we have shown that the pro-angiogenic effect of MBP is not due to its cationic charge since stimulation of the CAMs with the synthetic polycation, poly-L-arginine does not induce any angiogenic effects. CONCLUSIONS: These data demonstrate that MBP has pro-angiogenic effects in vitro and in vivo, providing a novel mechanism whereby MBP can participate in tissue inflammation and remodelling in atopic diseases.
allergic inflammation, angiogenesis, eosinophils, major basic protein, tissue remodelling
0105-4538
368-374
Puxeddu, I.
1fd52470-6cfa-4104-828b-cb7396265836
Berkman, N.
1553560f-11d1-4609-88d5-6d905cc66655
Ben Efrain, A.H. Nissin
490b7648-21f1-425e-b401-ed02cb267600
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Ribatti, D.
559d5189-8707-497a-b04e-f0d2dacd2240
Gleich, G.J.
fb303d24-fd52-4dd5-b211-fef4c0323b83
Levi-Schaffer, F.
a3ac91e9-7f28-43b0-a7cd-712e9f43fc94
Puxeddu, I.
1fd52470-6cfa-4104-828b-cb7396265836
Berkman, N.
1553560f-11d1-4609-88d5-6d905cc66655
Ben Efrain, A.H. Nissin
490b7648-21f1-425e-b401-ed02cb267600
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Ribatti, D.
559d5189-8707-497a-b04e-f0d2dacd2240
Gleich, G.J.
fb303d24-fd52-4dd5-b211-fef4c0323b83
Levi-Schaffer, F.
a3ac91e9-7f28-43b0-a7cd-712e9f43fc94

Puxeddu, I., Berkman, N., Ben Efrain, A.H. Nissin, Davies, D.E., Ribatti, D., Gleich, G.J. and Levi-Schaffer, F. (2009) The role of eosinophil major basic protein in angiogenesis. Allergy, 64 (3), 368-374. (doi:10.1111/j.1398-9995.2008.01822.x). (PMID:19120069)

Record type: Article

Abstract

BACKGROUND: Eosinophil-derived major basic protein (MBP) plays an active role in allergic inflammation and tissue remodelling. However, its role in angiogenesis has not been established as yet. Therefore our objective was to investigate whether MBP exhibits any direct pro-angiogenic effects. METHODS: Rat aortic endothelial cells and human umbilical vascular endothelial cells were cultured with different concentrations of MBP and their viability (Trypan blue exclusion test), proliferation (thymidine incorporation) and capillary-like structure formation (matrigel assay) were investigated in vitro. The angiogenic activity of MBP was then tested in vivo using the chick chorio allantoic membrane (CAM) assay. RESULTS: Subcytotoxic concentrations of MBP induce endothelial cell proliferation and enhance the pro-mitogenic effect of vascular endothelial growth factor (VEGF), but do not affect their VEGF release. MBP promotes capillarogenesis by endothelial cells seeded on matrigel and sprouting formation in the CAM assay. Furthermore, we have shown that the pro-angiogenic effect of MBP is not due to its cationic charge since stimulation of the CAMs with the synthetic polycation, poly-L-arginine does not induce any angiogenic effects. CONCLUSIONS: These data demonstrate that MBP has pro-angiogenic effects in vitro and in vivo, providing a novel mechanism whereby MBP can participate in tissue inflammation and remodelling in atopic diseases.

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More information

Published date: March 2009
Keywords: allergic inflammation, angiogenesis, eosinophils, major basic protein, tissue remodelling

Identifiers

Local EPrints ID: 70858
URI: http://eprints.soton.ac.uk/id/eprint/70858
ISSN: 0105-4538
PURE UUID: 3693f473-eae4-496d-83ac-34d46ee62844
ORCID for D.E. Davies: ORCID iD orcid.org/0000-0002-5117-2991

Catalogue record

Date deposited: 09 Feb 2010
Last modified: 14 Mar 2024 02:32

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Contributors

Author: I. Puxeddu
Author: N. Berkman
Author: A.H. Nissin Ben Efrain
Author: D.E. Davies ORCID iD
Author: D. Ribatti
Author: G.J. Gleich
Author: F. Levi-Schaffer

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