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World-to-chip interconnects for efficient loading of genomic DNA into microfluidic channels

World-to-chip interconnects for efficient loading of genomic DNA into microfluidic channels
World-to-chip interconnects for efficient loading of genomic DNA into microfluidic channels
A novel sloped interconnect for the efficient delivery of long genomic DNA fragments into a microfluidic channel is designed, fabricated and tested. Out-of-plane slopes are fabricated in silicon wafers using the deep reactive-ion etch lag phenomenon and a combination of anisotropic and isotropic etching. The final structure is capped with anodically bonded glass. Based upon a series of etch-lag calibration studies, the interconnect was designed using finite element analysis to provide a channel with flow acceleration properties appropriate to straighten DNA molecules. The efficiency of transit of the 48.5 kb DNA fragments (~16.5 µm long when fully extended) through the microfluidic device, established using quantitative real-time polymerase chain reaction, is 95 ± 7.3% .
0960-1317
105024-105032
Humphreys, Tim
6c34da06-31bf-4b3b-85db-69816f90033a
Andersson, Johan
4452b4e3-6af6-4f84-bfbf-9e719298c3d2
Sodervall, Ulf
8652f318-502b-4120-b444-b5d8330dde23
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Humphreys, Tim
6c34da06-31bf-4b3b-85db-69816f90033a
Andersson, Johan
4452b4e3-6af6-4f84-bfbf-9e719298c3d2
Sodervall, Ulf
8652f318-502b-4120-b444-b5d8330dde23
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Humphreys, Tim, Andersson, Johan, Sodervall, Ulf and Melvin, Tracy (2009) World-to-chip interconnects for efficient loading of genomic DNA into microfluidic channels. Journal of Micromechanics and Microengineering, 19 (10), 105024-105032. (doi:10.1088/0960-1317/19/10/105024).

Record type: Article

Abstract

A novel sloped interconnect for the efficient delivery of long genomic DNA fragments into a microfluidic channel is designed, fabricated and tested. Out-of-plane slopes are fabricated in silicon wafers using the deep reactive-ion etch lag phenomenon and a combination of anisotropic and isotropic etching. The final structure is capped with anodically bonded glass. Based upon a series of etch-lag calibration studies, the interconnect was designed using finite element analysis to provide a channel with flow acceleration properties appropriate to straighten DNA molecules. The efficiency of transit of the 48.5 kb DNA fragments (~16.5 µm long when fully extended) through the microfluidic device, established using quantitative real-time polymerase chain reaction, is 95 ± 7.3% .

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Published date: 22 September 2009

Identifiers

Local EPrints ID: 70952
URI: http://eprints.soton.ac.uk/id/eprint/70952
ISSN: 0960-1317
PURE UUID: 0ff395b8-0aad-44a9-be01-6f83bad4b849

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 04 Dec 2019 17:31

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