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Optical and impedance detection of DNA hybridisation on barcoded micro particles in a microfluidic device

Optical and impedance detection of DNA hybridisation on barcoded micro particles in a microfluidic device
Optical and impedance detection of DNA hybridisation on barcoded micro particles in a microfluidic device
A common problem with high throughput combinatorial chemistry is the inability to easily analyse and track the multitude of molecules produced. This bottleneck is a major problem for the big pharmaceutical companies and other researchers working in the fields of drug screening, DNA analysis, etc. To this end we have developed a microfluidic device capable of rapidly analysing the optical and impedance properties of individual polymer beads. Such beads are commonly used as solid supports for combi-chem and DNA synthesis. We have further developed the system by the use of barcoded microparticles. Each bead is uniquely identifiable by its optical diffraction pattern and can be tracked within the device allowing correlation between this unique code (which can be associated with its chemical history) and the optical and impedance measurements performed in the system.
dna hybridisation, barcoded particles, microfacs, on-chip flowcytometry, microfluidics
Holmes, D.
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Whitton, A.
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Cavalli-Petraglia, G.
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Galitonov, G.
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Birtwell, S.
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Banu, S.
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Zheludev, N.
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Morgan, H.
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Holmes, D.
0bd269a5-3bab-477c-8bb0-a8711a0aa14b
Whitton, A.
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Cavalli-Petraglia, G.
bca2eaf2-dadb-40de-a71b-c9f8456b1ec9
Galitonov, G.
604cc0ec-21b4-459b-b378-eb01dc63de2b
Birtwell, S.
b1d014b6-4286-49cf-925d-c33b409e4147
Banu, S.
ccf7dbae-dce6-4c1e-ab40-860cc3aee72a
Zheludev, N.
32fb6af7-97e4-4d11-bca6-805745e40cc6
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Holmes, D., Whitton, A., Cavalli-Petraglia, G., Galitonov, G., Birtwell, S., Banu, S., Zheludev, N. and Morgan, H. (2006) Optical and impedance detection of DNA hybridisation on barcoded micro particles in a microfluidic device. Nano Science and Technology Institute Nanotech - Conference on Nano Electronics & Photonics, United States. 07 - 11 May 2006.

Record type: Conference or Workshop Item (Paper)

Abstract

A common problem with high throughput combinatorial chemistry is the inability to easily analyse and track the multitude of molecules produced. This bottleneck is a major problem for the big pharmaceutical companies and other researchers working in the fields of drug screening, DNA analysis, etc. To this end we have developed a microfluidic device capable of rapidly analysing the optical and impedance properties of individual polymer beads. Such beads are commonly used as solid supports for combi-chem and DNA synthesis. We have further developed the system by the use of barcoded microparticles. Each bead is uniquely identifiable by its optical diffraction pattern and can be tracked within the device allowing correlation between this unique code (which can be associated with its chemical history) and the optical and impedance measurements performed in the system.

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More information

Published date: 9 May 2006
Venue - Dates: Nano Science and Technology Institute Nanotech - Conference on Nano Electronics & Photonics, United States, 2006-05-07 - 2006-05-11
Keywords: dna hybridisation, barcoded particles, microfacs, on-chip flowcytometry, microfluidics

Identifiers

Local EPrints ID: 70986
URI: https://eprints.soton.ac.uk/id/eprint/70986
PURE UUID: e0668d0d-415d-4d37-8cae-3e130308ccdf
ORCID for N. Zheludev: ORCID iD orcid.org/0000-0002-1013-6636
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 11 Dec 2009
Last modified: 20 Jul 2019 01:22

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Contributors

Author: D. Holmes
Author: A. Whitton
Author: G. Cavalli-Petraglia
Author: G. Galitonov
Author: S. Birtwell
Author: S. Banu
Author: N. Zheludev ORCID iD
Author: H. Morgan ORCID iD

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