Identification of lymphoma-association antigens using SEREX

Liggins, A., Guinn, B.A. and Banham, A.H. (2005) Identification of lymphoma-association antigens using SEREX. In, Illidge, Tim and Johnson, Peter W.M. (eds.) Lymphoma. (Methods in Molecular Medicine, 115) UK. Springer, pp. 109-128.

Abstract

SEREX (serological analysis of recombinant cDNA expression libraries) is a powerful approach to identify immunogenic cancer-associated proteins using antibodies naturally present in the serum of cancer patients. This technique involves the screening of a relevant cDNA expression library with patient serum that has been cleaned to remove any antibodies that may recognise bacterial and/or viral proteins. Once antigens have been identified and their reactivity has been confirmed with a second round of screening, the gene encoding the protein can be sequenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and normal control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full

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