The University of Southampton
University of Southampton Institutional Repository

Identification of lymphoma-association antigens using SEREX

Identification of lymphoma-association antigens using SEREX
Identification of lymphoma-association antigens using SEREX
SEREX (serological analysis of recombinant cDNA expression libraries) is a powerful approach to identify immunogenic cancer-associated proteins using antibodies naturally present in the serum of cancer patients. This technique involves the screening of a relevant cDNA expression library with patient serum that has been cleaned to remove any antibodies that may recognise bacterial and/or viral proteins. Once antigens have been identified and their reactivity has been confirmed with a second round of screening, the gene encoding the protein can be sequenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and normal control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full
SEREX (serological analysis of recombinant cDNA expression libraries), lymphoma, antigen, antibody, immune response, immunotherapy, expression cloning, serology, autologous serum, immunoscreening, cancer antigen
9781588291592
109-128
Springer
Liggins, A.
36e60f72-ebdc-4b2f-afb7-8f15a7c9785a
Guinn, B.A.
30273eb5-489d-4f00-816e-55d73a43b7c7
Banham, A.H.
6186fe6c-06b6-48ca-9c39-1759f3188834
Illidge, Tim
Johnson, Peter W.M.
Liggins, A.
36e60f72-ebdc-4b2f-afb7-8f15a7c9785a
Guinn, B.A.
30273eb5-489d-4f00-816e-55d73a43b7c7
Banham, A.H.
6186fe6c-06b6-48ca-9c39-1759f3188834
Illidge, Tim
Johnson, Peter W.M.

Liggins, A., Guinn, B.A. and Banham, A.H. (2005) Identification of lymphoma-association antigens using SEREX. In, Illidge, Tim and Johnson, Peter W.M. (eds.) Lymphoma. (Methods in Molecular Medicine, 115) UK. Springer, pp. 109-128.

Record type: Book Section

Abstract

SEREX (serological analysis of recombinant cDNA expression libraries) is a powerful approach to identify immunogenic cancer-associated proteins using antibodies naturally present in the serum of cancer patients. This technique involves the screening of a relevant cDNA expression library with patient serum that has been cleaned to remove any antibodies that may recognise bacterial and/or viral proteins. Once antigens have been identified and their reactivity has been confirmed with a second round of screening, the gene encoding the protein can be sequenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and normal control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full

This record has no associated files available for download.

More information

Published date: 2005
Keywords: SEREX (serological analysis of recombinant cDNA expression libraries), lymphoma, antigen, antibody, immune response, immunotherapy, expression cloning, serology, autologous serum, immunoscreening, cancer antigen

Identifiers

Local EPrints ID: 71574
URI: http://eprints.soton.ac.uk/id/eprint/71574
ISBN: 9781588291592
PURE UUID: a01b4e85-741c-47d1-a500-21eeb9e1a189

Catalogue record

Date deposited: 14 Dec 2009
Last modified: 22 Jul 2022 17:09

Export record

Contributors

Author: A. Liggins
Author: B.A. Guinn
Author: A.H. Banham
Editor: Tim Illidge
Editor: Peter W.M. Johnson

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×