Experimental–computational evaluation of human bone marrow stromal cell spreading on trabecular bone structures
Experimental–computational evaluation of human bone marrow stromal cell spreading on trabecular bone structures
The clinical application of macro-porous scaffolds for bone regeneration is significantly affected by the problem of insufficient cell colonization. Given the wide variety of different scaffold structures used for tissue engineering it is essential to derive relationships for cell colonization independent of scaffold architecture. To study cell population spreading on 3D structures decoupled from nutrient limitations, an in vitro culture system was developed consisting of thin slices of human trabecular bone seeded with Human Bone Marrow Stromal Cells, combined with dedicated ?CT imaging and computational modeling of cell population spreading. Only the first phase of in vitro scaffold colonization was addressed, in which cells migrate and proliferate up to the stage when the surface of the bone is covered as a monolayer, a critical prerequisite for further tissue formation. The results confirm the model’s ability to represent experimentally observed cell population spreading. The key advantage of the computational model was that by incorporating complex 3D structure, cell behavior can be characterized quantitatively in terms of intrinsic migration parameters, which could potentially be used for predictions on different macro-porous scaffolds subject to additional experimental validation. This type of modeling will prove useful in predicting cell colonization and improving strategies for skeletal tissue engineering.
skeletal tissue engineering, cell migration, scaffold architecture, computational modeling
1165-1176
Sengers, B.G.
d6b771b1-4ede-48c5-9644-fa86503941aa
Please, C.P.
118dffe7-4b38-4787-a972-9feec535839e
Taylor, M.
e368bda3-6ca5-4178-80e9-41a689badeeb
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
June 2009
Sengers, B.G.
d6b771b1-4ede-48c5-9644-fa86503941aa
Please, C.P.
118dffe7-4b38-4787-a972-9feec535839e
Taylor, M.
e368bda3-6ca5-4178-80e9-41a689badeeb
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Sengers, B.G., Please, C.P., Taylor, M. and Oreffo, R.O.C.
(2009)
Experimental–computational evaluation of human bone marrow stromal cell spreading on trabecular bone structures.
Annals of Biomedical Engineering, 37 (6), .
(doi:10.1007/s10439-009-9676-3).
Abstract
The clinical application of macro-porous scaffolds for bone regeneration is significantly affected by the problem of insufficient cell colonization. Given the wide variety of different scaffold structures used for tissue engineering it is essential to derive relationships for cell colonization independent of scaffold architecture. To study cell population spreading on 3D structures decoupled from nutrient limitations, an in vitro culture system was developed consisting of thin slices of human trabecular bone seeded with Human Bone Marrow Stromal Cells, combined with dedicated ?CT imaging and computational modeling of cell population spreading. Only the first phase of in vitro scaffold colonization was addressed, in which cells migrate and proliferate up to the stage when the surface of the bone is covered as a monolayer, a critical prerequisite for further tissue formation. The results confirm the model’s ability to represent experimentally observed cell population spreading. The key advantage of the computational model was that by incorporating complex 3D structure, cell behavior can be characterized quantitatively in terms of intrinsic migration parameters, which could potentially be used for predictions on different macro-porous scaffolds subject to additional experimental validation. This type of modeling will prove useful in predicting cell colonization and improving strategies for skeletal tissue engineering.
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Submitted date: 16 June 2008
Published date: June 2009
Keywords:
skeletal tissue engineering, cell migration, scaffold architecture, computational modeling
Organisations:
Bioengineering Sciences
Identifiers
Local EPrints ID: 71579
URI: http://eprints.soton.ac.uk/id/eprint/71579
ISSN: 0090-6964
PURE UUID: 08ce82ca-8fed-4b2f-81be-bf5fa8ebd31b
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Date deposited: 15 Dec 2009
Last modified: 14 Mar 2024 02:51
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Author:
C.P. Please
Author:
M. Taylor
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