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Denitrification in human dental plaque

Schreiber, F., Stief, P., Gieseke, A., Heisterkampa, I., Verstraete, W., DeBeer, D. and Stoodley, P. (2010) Denitrification in human dental plaque BMC Biology, 8, (24) (doi:10.1186/1741-7007-8-24).

Record type: Article

Abstract

Background
Microbial denitrification is not considered important in human-associated microbial communities. Accordingly, metabolic investigations of the microbial biofilm communities of human dental plaque have focused on aerobic respiration and acid fermentation of carbohydrates, even though it is known that the oral habitat is constantly exposed to nitrate (NO3-) concentrations in the millimolar range and that dental plaque houses bacteria that can reduce this NO3- to nitrite (NO2-).

Results
We show that dental plaque mediates denitrification of NO3- to nitric oxide (NO), nitrous oxide (N2O), and dinitrogen (N2) using microsensor measurements, 15N isotopic labelling and molecular detection of denitrification genes. In vivo N2O accumulation rates in the mouth depended on the presence of dental plaque and on salivary NO3- concentrations. NO and N2O production by denitrification occurred under aerobic conditions and was regulated by plaque pH.

Conclusions
Increases of NO concentrations were in the range of effective concentrations for NO signalling to human host cells and, thus, may locally affect blood flow, signalling between nerves and inflammatory processes in the gum. This is specifically significant for the understanding of periodontal diseases, where NO has been shown to play a key role, but where gingival cells are believed to be the only source of NO. More generally, this study establishes denitrification by human-associated microbial communities as a significant metabolic pathway which, due to concurrent NO formation, provides a basis for symbiotic interactions.

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More information

Published date: April 2010
Additional Information: The National Centre for Advanced Tribology at Southampton (nCATS)
Keywords: Denitrification as a source for nitric oxide and nitrous oxide in human dental plaque
Organisations: Engineering Mats & Surface Engineerg Gp

Identifiers

Local EPrints ID: 71665
URI: http://eprints.soton.ac.uk/id/eprint/71665
ISSN: 1471-2180
PURE UUID: f4c7b1bd-0b10-4268-aedc-34d286bd8792

Catalogue record

Date deposited: 17 Dec 2009
Last modified: 18 Jul 2017 23:59

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Contributors

Author: F. Schreiber
Author: P. Stief
Author: A. Gieseke
Author: I. Heisterkampa
Author: W. Verstraete
Author: D. DeBeer
Author: P. Stoodley

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