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Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

Wiedenmann, Joerg, Oswald, Franz and Nienhaus, Gerd Ulrich (2009) Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges IUMBM Life, 61, (11), pp. 1029-1042. (doi:10.1002/iub.256).

Record type: Article


The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes.

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Published date: 2009


Local EPrints ID: 71720
ISSN: 1521-6543
PURE UUID: 9b54f1ee-66f9-4834-8a3d-a827946ef943
ORCID for Joerg Wiedenmann: ORCID iD

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Date deposited: 18 Dec 2009
Last modified: 18 Jul 2017 23:59

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Author: Franz Oswald
Author: Gerd Ulrich Nienhaus

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