The University of Southampton
University of Southampton Institutional Repository

Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

Record type: Article

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes.

Full text not available from this repository.

Citation

Wiedenmann, Joerg, Oswald, Franz and Nienhaus, Gerd Ulrich (2009) Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges IUMBM Life, 61, (11), pp. 1029-1042. (doi:10.1002/iub.256).

More information

Published date: 2009

Identifiers

Local EPrints ID: 71720
URI: http://eprints.soton.ac.uk/id/eprint/71720
ISSN: 1521-6543
PURE UUID: 9b54f1ee-66f9-4834-8a3d-a827946ef943
ORCID for Joerg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

Catalogue record

Date deposited: 18 Dec 2009
Last modified: 18 Jul 2017 23:59

Export record

Altmetrics

Contributors

Author: Franz Oswald
Author: Gerd Ulrich Nienhaus

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×