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A Simple, Low-Cost Double Beam Spectrophotometer for Colorimetric Detection of Nitrite in Seawater

A Simple, Low-Cost Double Beam Spectrophotometer for Colorimetric Detection of Nitrite in Seawater
A Simple, Low-Cost Double Beam Spectrophotometer for Colorimetric Detection of Nitrite in Seawater
This paper reports on the optical detection system of a prototype nitrite sensor for seawater analysis. The sensor uses a colorimetric Griess reaction that produces an Azo dye with an absorption peak at 540 nm. A simple low-cost double beam spectrophotometer using a green light emitting diode (LED) light source, two integrated photo detectors and lock-in detection is described. A log-ratio amplifier is used to produce an output proportional to absorbance. The optical devices were mounted in a cast resin block to prevent shock and vibration. Experimental results show the detection system has a limit of detection of 0.1 muM.
9781424421251
1530-437X
862-869
Gong, Weidong
5e7fd497-7caa-4f9c-9b5e-b18e3abeecdb
Mowlem, Matt
6f633ca2-298f-48ee-a025-ce52dd62124f
Kraft, Michael
54927621-738f-4d40-af56-a027f686b59f
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Gong, Weidong
5e7fd497-7caa-4f9c-9b5e-b18e3abeecdb
Mowlem, Matt
6f633ca2-298f-48ee-a025-ce52dd62124f
Kraft, Michael
54927621-738f-4d40-af56-a027f686b59f
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Gong, Weidong, Mowlem, Matt, Kraft, Michael and Morgan, Hywel (2009) A Simple, Low-Cost Double Beam Spectrophotometer for Colorimetric Detection of Nitrite in Seawater. IEEE Sensors Journal, 9 (7), 862-869. (doi:10.1109/JSEN.2009.2020659).

Record type: Article

Abstract

This paper reports on the optical detection system of a prototype nitrite sensor for seawater analysis. The sensor uses a colorimetric Griess reaction that produces an Azo dye with an absorption peak at 540 nm. A simple low-cost double beam spectrophotometer using a green light emitting diode (LED) light source, two integrated photo detectors and lock-in detection is described. A log-ratio amplifier is used to produce an output proportional to absorbance. The optical devices were mounted in a cast resin block to prevent shock and vibration. Experimental results show the detection system has a limit of detection of 0.1 muM.

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More information

Published date: July 2009
Additional Information: Imported from ISI Web of Science
Organisations: Electronics & Computer Science, National Oceanography Centre,Southampton

Identifiers

Local EPrints ID: 71835
URI: https://eprints.soton.ac.uk/id/eprint/71835
ISBN: 9781424421251
ISSN: 1530-437X
PURE UUID: 0aed91bb-fb35-43db-8994-6d5ab37cff6e
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 04 Jan 2010
Last modified: 20 Jul 2019 00:59

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