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Microfluidic system for cell transfection using sonoporation and ultrasonic particle manipulation

Record type: Conference or Workshop Item (Paper)

Studies into sonoporation have grown rapidly in biotechnology and medicine in recent years. The use of sonoporation in biotechnology and medicine has potential for enhancing and targeting administration of drugs genes and other therapeutic compounds into the cells. Sonoporation has demonstrated to facilitate efficient gene transfer both in vitro and in vivo. This paper presents a microfluidic system for cells transfection using sonoporation and ultrasonic particle manipulation. The paper presents a theoretical analysis used to design the sonoporation chamber, whilst also enabling ultrasonic particle manipulation using an ultrasonic standing wave. Both the sonoporation and the particle manipulation are achieved using the same piezoelectric transducer (PZT 26) mounted on the micro chamber. The theoretical analysis is based on upon the model for an acoustic particle separator. The resulting design has been implemented in Macor ceramic glass assembly with a chamber size of 6 mm diameter and 750?m thickness. The efficiency of the sonoporation system was determined experimentally by the use of HeLa cells and propidium dye. The transfection rate was determined under a range of sonoporation conditions. The optimal sonoporation parameters will vary depending upon the cell type and purpose. For example, sonoporation typically utilises ultrasonic frequencies of 1 to 3MHz for gene delivery and drug delivery. The experiment results showed a peak cell transfection efficiency of 52.2% at 1.17MHz at 80 Vp-p

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Citation

Rodamporn, S., Beeby, S., Harris, N., Brown, A., Hill, M. and Chad, J. (2007) Microfluidic system for cell transfection using sonoporation and ultrasonic particle manipulation At International Conference on Cellular & Molecular Bioengineering. 10 - 12 Dec 2007.

More information

Published date: 2007
Venue - Dates: International Conference on Cellular & Molecular Bioengineering, 2007-12-10 - 2007-12-12

Identifiers

Local EPrints ID: 71846
URI: http://eprints.soton.ac.uk/id/eprint/71846
PURE UUID: 971584e6-e640-4eab-91dc-77152c7085f7
ORCID for S. Beeby: ORCID iD orcid.org/0000-0002-0800-1759
ORCID for N. Harris: ORCID iD orcid.org/0000-0003-4122-2219
ORCID for M. Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for J. Chad: ORCID iD orcid.org/0000-0001-6442-4281

Catalogue record

Date deposited: 05 Jan 2010
Last modified: 18 Jul 2017 23:58

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Contributors

Author: S. Rodamporn
Author: S. Beeby ORCID iD
Author: N. Harris ORCID iD
Author: A. Brown
Author: M. Hill ORCID iD
Author: J. Chad ORCID iD

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