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Cell-specific targeting in the mouse inner ear using nanoparticles conjugated with a neurotrophin-derived peptide ligand: potential tool for drug delivery

Record type: Article

Cell specific targeting is an emerging field in nanomedicine. Homing of the multifunctional nanoparticles (MFNPs) is achieved by the conjugation of targeting moieties on the nanoparticle surface. The inner ear is an attractive target for new drug delivery strategies as it is hard to access and hearing loss is a significant worldwide problem. In this work we investigated the utility of a Nerve Growth Factor-derived peptide (hNgf_EE) functionalized nanoparticles (NPs) to target cells of the inner ear. These functionalized NPs were introduced to organotypic explant cultures of the mouse inner ear and to PC-12 rat pheochromocytoma cells. The NPs did not show any signs of toxicity. Specific targeting and higher binding affinity to spiral ganglion neurons, Schwann cells and nerve fibers of the explant cultures were achieved through ligand mediated multivalent binding to tyrosine kinase receptors and to p75 neurotrophin receptors. Unspecific uptake of NPs was investigated using NPs conjugated with scrambled hNgf_EE peptide. Our results indicate a selective cochlear cell targeting by MFNPs, which may be a potential tool for cell specific drug and gene delivery to the inner ear

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Citation

Roy, Soumen, Johnston, Alex H., Newman, Tracey A., Glueckert, Rudolf, Dudas, Jozsef, Bitsche, Mario, Corbacella, Elisa, Rieger, Gunde, Martini, Alessandro and Fischer, Anneliese Schrott (2010) Cell-specific targeting in the mouse inner ear using nanoparticles conjugated with a neurotrophin-derived peptide ligand: potential tool for drug delivery International Journal of Pharmaceutics, 390, (2), pp. 214-224. (doi:10.1016/j.ijpharm.2010.02.003). (PMID:20153412).

More information

Submitted date: 25 November 2009
Accepted/In Press date: 25 November 2009
Published date: 10 May 2010
Keywords: inner ear, spiral ganglion neurons, nanoparticles, hearing loss
Organisations: Biological Sciences, Clinical Neurosciences

Identifiers

Local EPrints ID: 72704
URI: http://eprints.soton.ac.uk/id/eprint/72704
ISSN: 0378-5173
PURE UUID: 2f05e11c-8b27-4564-9476-6c936eb856de

Catalogue record

Date deposited: 19 Feb 2010
Last modified: 18 Jul 2017 23:53

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Contributors

Author: Soumen Roy
Author: Alex H. Johnston
Author: Rudolf Glueckert
Author: Jozsef Dudas
Author: Mario Bitsche
Author: Elisa Corbacella
Author: Gunde Rieger
Author: Alessandro Martini
Author: Anneliese Schrott Fischer

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