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Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry

Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microfluidic single cell impedance cytometer that performs a white blood cell differential count. The device consists of a microfluidic chip with micro-electrodes that measure the impedance of single cells at two frequencies. Human blood, treated with saponin/formic acid to lyse erythrocytes, flows through the device and a complete blood count is performed in a few minutes. Verification of cell dielectric parameters was performed by simultaneously measuring fluorescence from CD antibody-conjugated cells. This enabled direct correlation of impedance signals from individual cells with phenotype. Tests with patient samples showed 95% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system
1473-0197
2881-2889
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Pettigrew, David
907289d2-5f39-46d9-a4bb-20f9791a04df
Reccius, Christian H.
9bab1b54-e599-4c54-9d44-cc09a76f5d55
Gwyer, James D.
a996644a-37b0-4bc6-b47e-4e553f7c92a7
van Berkel, Cess
34086275-06d4-4ea1-bb48-8b74afc633dd
Holloway, Judith
f22f45f3-6fc8-4a4c-bc6c-24add507037c
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Pettigrew, David
907289d2-5f39-46d9-a4bb-20f9791a04df
Reccius, Christian H.
9bab1b54-e599-4c54-9d44-cc09a76f5d55
Gwyer, James D.
a996644a-37b0-4bc6-b47e-4e553f7c92a7
van Berkel, Cess
34086275-06d4-4ea1-bb48-8b74afc633dd
Holloway, Judith
f22f45f3-6fc8-4a4c-bc6c-24add507037c
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Holmes, David, Pettigrew, David, Reccius, Christian H., Gwyer, James D., van Berkel, Cess, Holloway, Judith, Davies, Donna E. and Morgan, Hywel (2009) Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry. Lab on a Chip, 9 (20), 2881-2889. (doi:10.1039/b910053a).

Record type: Article

Abstract

Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microfluidic single cell impedance cytometer that performs a white blood cell differential count. The device consists of a microfluidic chip with micro-electrodes that measure the impedance of single cells at two frequencies. Human blood, treated with saponin/formic acid to lyse erythrocytes, flows through the device and a complete blood count is performed in a few minutes. Verification of cell dielectric parameters was performed by simultaneously measuring fluorescence from CD antibody-conjugated cells. This enabled direct correlation of impedance signals from individual cells with phenotype. Tests with patient samples showed 95% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system

Full text not available from this repository.

More information

e-pub ahead of print date: 7 August 2009
Published date: October 2009
Organisations: Infection Inflammation & Immunity, Electronics & Computer Science

Identifiers

Local EPrints ID: 72744
URI: https://eprints.soton.ac.uk/id/eprint/72744
ISSN: 1473-0197
PURE UUID: d0f36e78-5cdb-4868-9215-3b321dbf8c91
ORCID for Judith Holloway: ORCID iD orcid.org/0000-0002-2268-3071
ORCID for Donna E. Davies: ORCID iD orcid.org/0000-0002-5117-2991
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 24 Feb 2010
Last modified: 10 Sep 2019 00:57

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