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Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry

Record type: Article

Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microfluidic single cell impedance cytometer that performs a white blood cell differential count. The device consists of a microfluidic chip with micro-electrodes that measure the impedance of single cells at two frequencies. Human blood, treated with saponin/formic acid to lyse erythrocytes, flows through the device and a complete blood count is performed in a few minutes. Verification of cell dielectric parameters was performed by simultaneously measuring fluorescence from CD antibody-conjugated cells. This enabled direct correlation of impedance signals from individual cells with phenotype. Tests with patient samples showed 95% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system

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Citation

Holmes, David, Pettigrew, David, Reccius, Christian H., Gwyer, James D., van Berkel, Cess, Holloway, Judith, Davies, Donna E. and Morgan, Hywel (2009) Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry Lab on a Chip, 9, (20), pp. 2881-2889. (doi:10.1039/b910053a).

More information

e-pub ahead of print date: 7 August 2009
Published date: October 2009
Organisations: Infection Inflammation & Immunity, Electronics & Computer Science

Identifiers

Local EPrints ID: 72744
URI: http://eprints.soton.ac.uk/id/eprint/72744
ISSN: 1473-0197
PURE UUID: d0f36e78-5cdb-4868-9215-3b321dbf8c91
ORCID for Judith Holloway: ORCID iD orcid.org/0000-0002-2268-3071
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 24 Feb 2010
Last modified: 18 Jul 2017 23:53

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Contributors

Author: David Holmes
Author: David Pettigrew
Author: Christian H. Reccius
Author: James D. Gwyer
Author: Cess van Berkel
Author: Judith Holloway ORCID iD
Author: Donna E. Davies
Author: Hywel Morgan ORCID iD

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