Delivery by Caesarean section, rather than vaginal delivery, promotes hepatic steatosis in piglets
Delivery by Caesarean section, rather than vaginal delivery, promotes hepatic steatosis in piglets
There has been a marked increase in the number of babies born by elective CS (Caesarean section). Following CS, the lack of normal stimuli that occur at birth alters the thermogeneic response, but any effects on hepatic metabolism have not been identified. In the present study, we compared the effect of delivery on hepatic metabolism in piglets, born either by CS or VD (vaginal delivery) and fed by TPN (total parenteral nutrition), by measuring lipid metabolism and enzyme activity coupled with metabolomic and genomic approaches. Hepatic lipid in the CS piglets at 7 days post-partum was in excess of 5 mg/g of liver consistent with hepatic steatosis, whereas in the VD piglets the amount of lipid was markedly lower (3 mg/g of liver) and below the threshold for a diagnosis of steatosis. Metabolomic analysis indicated that CS resulted in higher hepatic glycerol and lower glycerol phosphate dehydrogenase activity, suggesting that CS causes a decrease in hepatic gluconeogenesis from glycerol. CS also resulted in altered cholesterol handling and gene expression, despite the same dietary intake for 7 days post-partum. Furthermore, the CS piglets had a lower expression of interferon-responsive genes, but a higher expression of markers of immature hepatocytes. In conclusion, the results suggest that VD promotes normal liver maturation and hepatic metabolism, thereby reducing the accumulation of hepatic lipid.
birth, caesarean section, liver, metabolic syndrome, steatosis, total parenteral nutrition (tpn)
47-59
Hyde, Matthew J.
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Griffin, Julian L.
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Herrera, Emilio
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Byrne, Christopher D.
1370b997-cead-4229-83a7-53301ed2a43c
Clarke, Lynne
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Kemp, Paul R.
445abca1-22a2-41df-b809-4343cc2845be
January 2010
Hyde, Matthew J.
25aa8aa7-3b54-45d2-9bd5-006d1f98c839
Griffin, Julian L.
efbf2925-ee1c-4e28-8e89-c1a70d143928
Herrera, Emilio
cb590c3e-0a36-4a5d-9ca9-f5d757d77b99
Byrne, Christopher D.
1370b997-cead-4229-83a7-53301ed2a43c
Clarke, Lynne
4df39b1d-8d30-446c-b88e-f7d002f1f720
Kemp, Paul R.
445abca1-22a2-41df-b809-4343cc2845be
Hyde, Matthew J., Griffin, Julian L., Herrera, Emilio, Byrne, Christopher D., Clarke, Lynne and Kemp, Paul R.
(2010)
Delivery by Caesarean section, rather than vaginal delivery, promotes hepatic steatosis in piglets.
Clinical Science, 118 (1), .
(doi:10.1042/CS20090169).
Abstract
There has been a marked increase in the number of babies born by elective CS (Caesarean section). Following CS, the lack of normal stimuli that occur at birth alters the thermogeneic response, but any effects on hepatic metabolism have not been identified. In the present study, we compared the effect of delivery on hepatic metabolism in piglets, born either by CS or VD (vaginal delivery) and fed by TPN (total parenteral nutrition), by measuring lipid metabolism and enzyme activity coupled with metabolomic and genomic approaches. Hepatic lipid in the CS piglets at 7 days post-partum was in excess of 5 mg/g of liver consistent with hepatic steatosis, whereas in the VD piglets the amount of lipid was markedly lower (3 mg/g of liver) and below the threshold for a diagnosis of steatosis. Metabolomic analysis indicated that CS resulted in higher hepatic glycerol and lower glycerol phosphate dehydrogenase activity, suggesting that CS causes a decrease in hepatic gluconeogenesis from glycerol. CS also resulted in altered cholesterol handling and gene expression, despite the same dietary intake for 7 days post-partum. Furthermore, the CS piglets had a lower expression of interferon-responsive genes, but a higher expression of markers of immature hepatocytes. In conclusion, the results suggest that VD promotes normal liver maturation and hepatic metabolism, thereby reducing the accumulation of hepatic lipid.
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Published date: January 2010
Keywords:
birth, caesarean section, liver, metabolic syndrome, steatosis, total parenteral nutrition (tpn)
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Local EPrints ID: 72753
URI: http://eprints.soton.ac.uk/id/eprint/72753
ISSN: 0143-5221
PURE UUID: 7a9725d5-56bc-4998-b382-3dfc167fdae9
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Date deposited: 22 Feb 2010
Last modified: 14 Mar 2024 02:43
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Author:
Matthew J. Hyde
Author:
Julian L. Griffin
Author:
Emilio Herrera
Author:
Lynne Clarke
Author:
Paul R. Kemp
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