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Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring

Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
Background :miRNAs play important roles in the regulation of gene functions. Maternal dietary modifications during pregnancy and gestation have long-term effects on the offspring, but it is not known whether a maternal high fat (HF) diet during pregnancy and lactation alters expression of key miRNAs in the offspring.

Results: We studied the effects of maternal HF diet on the adult offspring by feeding mice with either a HF or a chow diet prior to conception, during pregnancy and lactation, and all offspring were weaned onto the same chow diet until adulthood. Maternal HF fed offspring had markedly increased hepatic mRNA levels of peroxisome proliferator activated receptor-alpha (ppar-alpha) and carnitine palmitoyl transferase-1a (cpt-1a) as well as insulin like growth factor-2 (Igf2). A HF diet induced up-regulation of ppar-alpha and cpt-1a expression in the wild type but not in Igf2 knock out mice. Furthermore, hepatic expression of let-7c was also reduced in maternal HF fed offspring. Among 579 miRNAs measured with microarray, ~23 miRNA levels were reduced by ~1.5-4.9-fold. Reduced expression of miR-709 (a highly expressed miRNA), miR-122, miR-192, miR-194, miR-26a, let-7a, let7b and let-7c, miR-494 and miR-483* (reduced by ~4.9 fold) was validated by qPCR. We found that methyl-CpG binding protein 2 was the common predicted target for miR-709, miR-let7s, miR-122, miR-194 and miR-26a using our own purpose-built computer program.

Conclusion: Maternal HF feeding during pregnancy and lactation induced co-ordinated and long-lasting changes in expression of Igf2, fat metabolic genes and several important miRNAs in the offspring.
1471-2164
1-12
Zhang, Junlong
68a8fa77-c5db-4b34-aa9a-fbad6860155f
Zhang, Fang
b13ed7af-a94f-411a-b220-ed332ca724b6
Didelot, Xavier
2d6849fa-f7a4-421d-a328-bacfc713af37
Bruce, Kimberley D.
2065fe20-0e9e-4f1a-8463-71b832776fa1
Cagampang, Felino R.
7cf57d52-4a65-4554-8306-ed65226bc50e
Vatish, Manu
ac1a9892-c0c2-49c7-b835-12173afdfe50
Hanson, Mark
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lehnert, Hedrik
d93e4465-92f3-4e09-b994-f75dc73b1d59
Ceriello, Antonio
bc758779-acf0-4634-b729-f2f302b7c2ef
Byrne, Christopher D.
1370b997-cead-4229-83a7-53301ed2a43c
Zhang, Junlong
68a8fa77-c5db-4b34-aa9a-fbad6860155f
Zhang, Fang
b13ed7af-a94f-411a-b220-ed332ca724b6
Didelot, Xavier
2d6849fa-f7a4-421d-a328-bacfc713af37
Bruce, Kimberley D.
2065fe20-0e9e-4f1a-8463-71b832776fa1
Cagampang, Felino R.
7cf57d52-4a65-4554-8306-ed65226bc50e
Vatish, Manu
ac1a9892-c0c2-49c7-b835-12173afdfe50
Hanson, Mark
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lehnert, Hedrik
d93e4465-92f3-4e09-b994-f75dc73b1d59
Ceriello, Antonio
bc758779-acf0-4634-b729-f2f302b7c2ef
Byrne, Christopher D.
1370b997-cead-4229-83a7-53301ed2a43c

Zhang, Junlong, Zhang, Fang, Didelot, Xavier, Bruce, Kimberley D., Cagampang, Felino R., Vatish, Manu, Hanson, Mark, Lehnert, Hedrik, Ceriello, Antonio and Byrne, Christopher D. (2009) Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring. BMC Genomics, 10 (478), 1-12. (doi:10.1186/1471-2164-10-478).

Record type: Article

Abstract

Background :miRNAs play important roles in the regulation of gene functions. Maternal dietary modifications during pregnancy and gestation have long-term effects on the offspring, but it is not known whether a maternal high fat (HF) diet during pregnancy and lactation alters expression of key miRNAs in the offspring.

Results: We studied the effects of maternal HF diet on the adult offspring by feeding mice with either a HF or a chow diet prior to conception, during pregnancy and lactation, and all offspring were weaned onto the same chow diet until adulthood. Maternal HF fed offspring had markedly increased hepatic mRNA levels of peroxisome proliferator activated receptor-alpha (ppar-alpha) and carnitine palmitoyl transferase-1a (cpt-1a) as well as insulin like growth factor-2 (Igf2). A HF diet induced up-regulation of ppar-alpha and cpt-1a expression in the wild type but not in Igf2 knock out mice. Furthermore, hepatic expression of let-7c was also reduced in maternal HF fed offspring. Among 579 miRNAs measured with microarray, ~23 miRNA levels were reduced by ~1.5-4.9-fold. Reduced expression of miR-709 (a highly expressed miRNA), miR-122, miR-192, miR-194, miR-26a, let-7a, let7b and let-7c, miR-494 and miR-483* (reduced by ~4.9 fold) was validated by qPCR. We found that methyl-CpG binding protein 2 was the common predicted target for miR-709, miR-let7s, miR-122, miR-194 and miR-26a using our own purpose-built computer program.

Conclusion: Maternal HF feeding during pregnancy and lactation induced co-ordinated and long-lasting changes in expression of Igf2, fat metabolic genes and several important miRNAs in the offspring.

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More information

Published date: 16 October 2009
Organisations: Faculty of Medicine, Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 72776
URI: https://eprints.soton.ac.uk/id/eprint/72776
ISSN: 1471-2164
PURE UUID: 1513d49b-05b3-4246-a7a3-c825f4921803
ORCID for Felino R. Cagampang: ORCID iD orcid.org/0000-0003-4404-9853
ORCID for Mark Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for Christopher D. Byrne: ORCID iD orcid.org/0000-0001-6322-7753

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Date deposited: 22 Feb 2010
Last modified: 11 Dec 2018 01:34

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