Osteopontin is expressed and functional in human eosinophils
Osteopontin is expressed and functional in human eosinophils
BACKGROUND: Eosinophils are critically involved in allergic inflammation and tissue remodeling. Osteopontin (OPN) is a glycoprotein molecule which exhibits pro-fibrogenic and pro-angiogenic properties and has recently also been implicated in allergic diseases. In this study, we investigated the expression and function of OPN in human eosinophils. METHODS: Osteopontin mRNA (RT-PCR) and protein (immunofluorescence) expression in peripheral blood eosinophils from atopic human subjects were evaluated. Soluble OPN release was determined in resting and activated eosinophils. The contribution of OPN to eosinophil-induced angiogenesis was determined using the chick embryo chorio- allantoic membrane (CAM) assay and OPN-induced eosinophil chemotaxis was determined (ChemoTx System microplate wells). Finally, OPN expression in bronchoalveolar lavage (BAL) fluids from mild asthmatic and normal control subjects was determined. RESULTS: Osteopontin is expressed in human eosinophils and is increased following GM-CSF and IL-5 activation. Eosinophil-derived OPN contributes to eosinophil-induced angiogenesis. Recombinant OPN promotes eosinophil chemotaxis in vitro and this effect is mediated by alpha(4)beta(1) integrin binding. Soluble OPN is increased in the bronchoalveolar lavage fluid from mild asthmatic subjects and correlates with eosinophil counts. CONCLUSIONS: We therefore conclude that OPN is likely to contribute to the process of angiogenesis observed in the airways in asthma
168-174
Puxeddu, I.
1fd52470-6cfa-4104-828b-cb7396265836
Berkman, D.
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Ribatti, R.
f9edaee2-c44d-4c12-9e78-a249cd0645b3
Bader, H.
851e8b54-fc4a-4ce1-a138-fdc99951597e
Haitchi, H.M.
68dadb29-305d-4236-884f-e9c93f4d78fe
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Levi-Schaffer, F.
a3ac91e9-7f28-43b0-a7cd-712e9f43fc94
February 2010
Puxeddu, I.
1fd52470-6cfa-4104-828b-cb7396265836
Berkman, D.
cd4493cb-7baa-488c-a252-149eb45003c9
Ribatti, R.
f9edaee2-c44d-4c12-9e78-a249cd0645b3
Bader, H.
851e8b54-fc4a-4ce1-a138-fdc99951597e
Haitchi, H.M.
68dadb29-305d-4236-884f-e9c93f4d78fe
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Levi-Schaffer, F.
a3ac91e9-7f28-43b0-a7cd-712e9f43fc94
Puxeddu, I., Berkman, D., Ribatti, R., Bader, H., Haitchi, H.M., Davies, D.E., Howarth, P.H. and Levi-Schaffer, F.
(2010)
Osteopontin is expressed and functional in human eosinophils.
Allergy, 65 (2), .
(doi:10.1111/j.1398-9995.2009.02148.x).
Abstract
BACKGROUND: Eosinophils are critically involved in allergic inflammation and tissue remodeling. Osteopontin (OPN) is a glycoprotein molecule which exhibits pro-fibrogenic and pro-angiogenic properties and has recently also been implicated in allergic diseases. In this study, we investigated the expression and function of OPN in human eosinophils. METHODS: Osteopontin mRNA (RT-PCR) and protein (immunofluorescence) expression in peripheral blood eosinophils from atopic human subjects were evaluated. Soluble OPN release was determined in resting and activated eosinophils. The contribution of OPN to eosinophil-induced angiogenesis was determined using the chick embryo chorio- allantoic membrane (CAM) assay and OPN-induced eosinophil chemotaxis was determined (ChemoTx System microplate wells). Finally, OPN expression in bronchoalveolar lavage (BAL) fluids from mild asthmatic and normal control subjects was determined. RESULTS: Osteopontin is expressed in human eosinophils and is increased following GM-CSF and IL-5 activation. Eosinophil-derived OPN contributes to eosinophil-induced angiogenesis. Recombinant OPN promotes eosinophil chemotaxis in vitro and this effect is mediated by alpha(4)beta(1) integrin binding. Soluble OPN is increased in the bronchoalveolar lavage fluid from mild asthmatic subjects and correlates with eosinophil counts. CONCLUSIONS: We therefore conclude that OPN is likely to contribute to the process of angiogenesis observed in the airways in asthma
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Published date: February 2010
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Local EPrints ID: 72801
URI: http://eprints.soton.ac.uk/id/eprint/72801
ISSN: 0105-4538
PURE UUID: 7a734c3b-916c-42be-a1a1-60d9e2f6f84d
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Date deposited: 23 Feb 2010
Last modified: 14 Mar 2024 02:47
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Author:
I. Puxeddu
Author:
D. Berkman
Author:
R. Ribatti
Author:
H. Bader
Author:
F. Levi-Schaffer
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