Dynamic lipidomics with stable isotope labeling
Dynamic lipidomics with stable isotope labeling
Incorporation of stable isotope labelled precursors enables estimation of the kinetics of lipid synthesis and turnover (dynamic lipidomics) in the clinical as well the experimental setting. Recent advances in tandem mass spectrometry extend the analytical possibilities from measurements of isotope enrichments to determinations of intact substrates. Incorporations of deuteriated choline, ethanolamine and inositol can be determined by precursor and neutral loss scans of phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol, respectively. This experimental approach provides information on the kinetics of individual phospholipid molecular species and has considerable potential to probe diseases of lipid metabolism in vivo.
2716-2721
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Hunt, Alan N.
95a3e223-da96-40e7-b47d-27dce014e305
September 2009
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Hunt, Alan N.
95a3e223-da96-40e7-b47d-27dce014e305
Postle, Anthony D. and Hunt, Alan N.
(2009)
Dynamic lipidomics with stable isotope labeling.
Journal of Chromatography B: Biomedical Sciences and Applications, 877 (26), .
(doi:10.1016/j.jchromb.2009.03.046).
Abstract
Incorporation of stable isotope labelled precursors enables estimation of the kinetics of lipid synthesis and turnover (dynamic lipidomics) in the clinical as well the experimental setting. Recent advances in tandem mass spectrometry extend the analytical possibilities from measurements of isotope enrichments to determinations of intact substrates. Incorporations of deuteriated choline, ethanolamine and inositol can be determined by precursor and neutral loss scans of phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol, respectively. This experimental approach provides information on the kinetics of individual phospholipid molecular species and has considerable potential to probe diseases of lipid metabolism in vivo.
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Published date: September 2009
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Local EPrints ID: 72861
URI: http://eprints.soton.ac.uk/id/eprint/72861
PURE UUID: fc59eaf4-2a73-47fb-8805-ad2f235930cd
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Date deposited: 24 Feb 2010
Last modified: 14 Mar 2024 02:38
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Author:
Alan N. Hunt
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