Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability
Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability
Environmental particulates impact first on airway epithelium, whereas circulating infiltrating cells are recruited through the underlying endothelium. An effective cellular immune response requires coordination between endothelium and epithelium. The authors have developed a bilayer culture model consisting of human bronchial epithelial derived cells (16HBE 14o-) and human umbilical vein endothelial cells (HUVECs) cultured as confluent layers on either side of a porous membrane. Confocal microscopy with epithelial and endothelial-specific antibodies showed segregated cell layers. By scanning and transmission electron microscopy, both cell types are polarized and tight junctions formed at the apical interface between cells. Epithelial cells grown in a bilayer showed significantly increased transepithelial resistance (TER) of 2260 +/- 64 Omega.cm(2) compared to epithelial or endothelial monolayers alone (1400 +/- 70 or 80 +/- 12 Omega.cm(2), respectively). This reflected decreased permeability and was unrelated to cell density or height. Increased TER coincided with increased occludin mRNA and protein in the epithelial cell layer as determined by polymerase chain reaction (PCR) and immunoblotting. Conditioned medium showed that decreased permeability was mediated by soluble endothelial-derived factor(s). This model reflects the in vivo relationship of human airway endothelial cells and epithelial cells. Altered tight junction permeability in cocultures indicates that these cells can work together as an active part of the mucosal barrier.
bilayer model, coculture, HEA-125, occludin, permeability, tight junctions, vWF
1-11
Chowdhury, Ferdousi
0af499d4-17c5-40cf-9426-0d509ab82595
Howat, William J.
3e5963d4-8992-4768-9b9d-0e3ec317510b
Phillips, Gary J.
8ceaaf43-e24f-4774-9e57-740d36e676ac
Lackie, Peter M.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
February 2010
Chowdhury, Ferdousi
0af499d4-17c5-40cf-9426-0d509ab82595
Howat, William J.
3e5963d4-8992-4768-9b9d-0e3ec317510b
Phillips, Gary J.
8ceaaf43-e24f-4774-9e57-740d36e676ac
Lackie, Peter M.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Chowdhury, Ferdousi, Howat, William J., Phillips, Gary J. and Lackie, Peter M.
(2010)
Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.
Experimental Lung Research, 36 (1), .
(doi:10.3109/01902140903026582).
Abstract
Environmental particulates impact first on airway epithelium, whereas circulating infiltrating cells are recruited through the underlying endothelium. An effective cellular immune response requires coordination between endothelium and epithelium. The authors have developed a bilayer culture model consisting of human bronchial epithelial derived cells (16HBE 14o-) and human umbilical vein endothelial cells (HUVECs) cultured as confluent layers on either side of a porous membrane. Confocal microscopy with epithelial and endothelial-specific antibodies showed segregated cell layers. By scanning and transmission electron microscopy, both cell types are polarized and tight junctions formed at the apical interface between cells. Epithelial cells grown in a bilayer showed significantly increased transepithelial resistance (TER) of 2260 +/- 64 Omega.cm(2) compared to epithelial or endothelial monolayers alone (1400 +/- 70 or 80 +/- 12 Omega.cm(2), respectively). This reflected decreased permeability and was unrelated to cell density or height. Increased TER coincided with increased occludin mRNA and protein in the epithelial cell layer as determined by polymerase chain reaction (PCR) and immunoblotting. Conditioned medium showed that decreased permeability was mediated by soluble endothelial-derived factor(s). This model reflects the in vivo relationship of human airway endothelial cells and epithelial cells. Altered tight junction permeability in cocultures indicates that these cells can work together as an active part of the mucosal barrier.
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Published date: February 2010
Keywords:
bilayer model, coculture, HEA-125, occludin, permeability, tight junctions, vWF
Identifiers
Local EPrints ID: 72909
URI: http://eprints.soton.ac.uk/id/eprint/72909
ISSN: 0190-2148
PURE UUID: 10b62852-67c3-4cc9-925f-7381f4fe8cbe
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Date deposited: 24 Feb 2010
Last modified: 14 Mar 2024 02:37
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Author:
Ferdousi Chowdhury
Author:
William J. Howat
Author:
Gary J. Phillips
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