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Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO–cGMP-dependent memory formation

Record type: Article

In a number of neuronal models of learning signalling by endogenous nitric oxide (NO), produced by the enzyme NO synthase (NOS), is essential for the formation of long-term memory (LTM). For example, in the molluscan model system Lymnaea, NO is required for LTM formation in the first few hours after one-trial reward conditioning. Furthermore, conditioning leads to transient up-regulation of the NOS gene in identified modulatory neurons, the cerebral giant cells (CGCs), which are known to be involved in LTM formation. In Lymnaea nothing is known however about the structure and localization of the major receptor for NO, the soluble guanylyl cyclase (sGC). Here we report on the cloning and characterization of both ? and ? subunits of NO-sensitive sGC and show that they are coexpressed in the CGCs. Furthermore, our electrophysiological experiments on isolated CGCs show that these neurons respond to NO by generating a prolonged depolarization of the membrane potential. Moreover, we demonstrate that this depolarization is blocked by ODQ, supporting our hypothesis that it is mediated by sGC.

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Citation

Riberio, Maria, Straub, Volko A., Schofield, Michael, Picot, Jo, Benjamin, Paul R., O'Shea, Michael and Korneev, Sergei A. (2008) Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO–cGMP-dependent memory formation European Journal of Neuroscience, 28, (6), pp. 1157-1165. (doi:10.1111/j.1460-9568.2008.06416.x).

More information

Published date: September 2008
Keywords: cerebral giant cell, identified neuron, Lymnaea, mollusc, nitric oxide

Identifiers

Local EPrints ID: 72919
URI: http://eprints.soton.ac.uk/id/eprint/72919
ISSN: 0953-816X
PURE UUID: 144a2ebb-132b-412d-b1e0-2570f1229bf9
ORCID for Jo Picot: ORCID iD orcid.org/0000-0001-5987-996X

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Date deposited: 25 Feb 2010
Last modified: 18 Jul 2017 23:52

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Contributors

Author: Maria Riberio
Author: Volko A. Straub
Author: Michael Schofield
Author: Jo Picot ORCID iD
Author: Paul R. Benjamin
Author: Michael O'Shea
Author: Sergei A. Korneev

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