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Opa protein repertoires of disease-causing and carried meningococci.

Opa protein repertoires of disease-causing and carried meningococci.
Opa protein repertoires of disease-causing and carried meningococci.
The meningococcal Opa proteins play an important role in pathogenesis by mediating invasion of human cells. The aim of this investigation was to determine whether carried and disease-associated meningococci possess different Opa repertoires and whether the diversity of these proteins is associated with clinical severity of disease. Opa repertoires in 227 disease-associated meningococci, isolated in the United Kingdom over a period of 6 years, were compared to the repertoires in 190 asymptomatically carried meningococci isolated in the United Kingdom from a contemporary, nonepidemic period. Multidimensional scaling (MDS) was employed to investigate the association between Opa repertoires and multilocus sequence typing (MLST) genotypes. Associations with clinical severity were also analyzed statistically. High levels of diversity were observed in opa alleles, variable regions, and repertoires, and MDS revealed that MLST genotypes were strongly associated with particular Opa repertoires. Individual Opa proteins or repertoires were not associated with clinical severity, though there was a trend toward an association with the opaD locus. Meningococcal Opa repertoire is strongly linked to MLST genotype irrespective of epidemiological sampling and therefore correlates with invasiveness. It is not, however, strongly associated with severity of meningococcal disease
0095-1137
3033-3041
Callaghan, Martin J.
dfa01e56-a803-4c5c-927a-645ca405acda
Buckee, Caroline
6955fc06-e3b3-4ed3-b133-70306f705964
McCarthy, Noel D.
18d342dc-bd89-4c54-b191-252b4cda555a
Ibarz Pavon, Ana Belan
29f2aacb-785d-458b-b05a-9a4fc8e9a023
Jolley, Keith A.
4ae51ae9-7a80-4ce0-a61c-1828b602d2ac
Faust, Saul
f97df780-9f9b-418e-b349-7adf63e150c1
Gray, Stephen J.
700f5c3e-b904-4ea4-a22c-6c5e28539358
Kaczmarski, Edward B.
b64d93b8-aaee-44f6-a7a3-715f8971d3b3
Levin, Michael
2be78359-2a08-4885-a83c-4f7a6d1de986
Kroll, J. Simon
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Maiden, Martin C.J.
1c2e2dea-365a-4ee8-af41-31e1949de442
Pollard, Andrew J.
f54083f3-c730-4ecb-937e-6fb11fdd6a21
Callaghan, Martin J.
dfa01e56-a803-4c5c-927a-645ca405acda
Buckee, Caroline
6955fc06-e3b3-4ed3-b133-70306f705964
McCarthy, Noel D.
18d342dc-bd89-4c54-b191-252b4cda555a
Ibarz Pavon, Ana Belan
29f2aacb-785d-458b-b05a-9a4fc8e9a023
Jolley, Keith A.
4ae51ae9-7a80-4ce0-a61c-1828b602d2ac
Faust, Saul
f97df780-9f9b-418e-b349-7adf63e150c1
Gray, Stephen J.
700f5c3e-b904-4ea4-a22c-6c5e28539358
Kaczmarski, Edward B.
b64d93b8-aaee-44f6-a7a3-715f8971d3b3
Levin, Michael
2be78359-2a08-4885-a83c-4f7a6d1de986
Kroll, J. Simon
ba664a5e-134c-48d9-8e2f-a656241d4b23
Maiden, Martin C.J.
1c2e2dea-365a-4ee8-af41-31e1949de442
Pollard, Andrew J.
f54083f3-c730-4ecb-937e-6fb11fdd6a21

Callaghan, Martin J., Buckee, Caroline, McCarthy, Noel D., Ibarz Pavon, Ana Belan, Jolley, Keith A., Faust, Saul, Gray, Stephen J., Kaczmarski, Edward B., Levin, Michael, Kroll, J. Simon, Maiden, Martin C.J. and Pollard, Andrew J. (2008) Opa protein repertoires of disease-causing and carried meningococci. Journal of Clinical Microbiology, 46 (9), 3033-3041. (doi:10.1128/JCM.00005-08).

Record type: Article

Abstract

The meningococcal Opa proteins play an important role in pathogenesis by mediating invasion of human cells. The aim of this investigation was to determine whether carried and disease-associated meningococci possess different Opa repertoires and whether the diversity of these proteins is associated with clinical severity of disease. Opa repertoires in 227 disease-associated meningococci, isolated in the United Kingdom over a period of 6 years, were compared to the repertoires in 190 asymptomatically carried meningococci isolated in the United Kingdom from a contemporary, nonepidemic period. Multidimensional scaling (MDS) was employed to investigate the association between Opa repertoires and multilocus sequence typing (MLST) genotypes. Associations with clinical severity were also analyzed statistically. High levels of diversity were observed in opa alleles, variable regions, and repertoires, and MDS revealed that MLST genotypes were strongly associated with particular Opa repertoires. Individual Opa proteins or repertoires were not associated with clinical severity, though there was a trend toward an association with the opaD locus. Meningococcal Opa repertoire is strongly linked to MLST genotype irrespective of epidemiological sampling and therefore correlates with invasiveness. It is not, however, strongly associated with severity of meningococcal disease

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Published date: September 2008

Identifiers

Local EPrints ID: 72967
URI: https://eprints.soton.ac.uk/id/eprint/72967
ISSN: 0095-1137
PURE UUID: e90ea30c-cb3b-4170-bc56-5069a0e175ad
ORCID for Saul Faust: ORCID iD orcid.org/0000-0003-3410-7642

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Date deposited: 25 Feb 2010
Last modified: 06 Jun 2018 12:40

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Contributors

Author: Martin J. Callaghan
Author: Caroline Buckee
Author: Noel D. McCarthy
Author: Ana Belan Ibarz Pavon
Author: Keith A. Jolley
Author: Saul Faust ORCID iD
Author: Stephen J. Gray
Author: Edward B. Kaczmarski
Author: Michael Levin
Author: J. Simon Kroll
Author: Martin C.J. Maiden
Author: Andrew J. Pollard

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