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Differential expression of claudin tight junction proteins in the human cortical nephron

Differential expression of claudin tight junction proteins in the human cortical nephron
Differential expression of claudin tight junction proteins in the human cortical nephron
Background: in renal tubules, paracellular permeability is tightly controlled to facilitate solute absorption and urinary concentration and is regulated by tight junctions, which incorporate claudin proteins. There is very limited information confirming the localization of these proteins in the human renal cortex. Most data is inferred from mouse, bovine and rabbit studies and differences exist between mouse and other species.

Methods: a survey of claudin staining was performed on human kidney cortex embedded in glycolmethacrylate resin to enhance tissue morphology and facilitate the cutting of 2 µm serial sections.

Results: claudin-2, -10 and -11 antibodies labelled renal tubular epithelial cells, correlating with Lotus tetragonolobus and N-cadherin positive proximal tubules. Claudin-3, -10, -11 and -16 antibodies strongly stained a population of tubules that were positive for Tamm Horsfall protein on adjacent sections, confirming expression in the thick ascending limb of the Loop of Henle. Claudin-3, -4 and -8 antibodies reacted with tubules that correlated with the distal nephron markers, E-cadherin, epithelial membrane antigen and Dolichos biflorus and claudin-3, -4, -7 and -8 with the distal tubule marker, calbindin, and the collecting duct marker, aquaporin-2. Claudin-14 was localized in distal convoluted tubules, correlating positively with calbindin but negatively with aquaporin-2, whereas claudin-1 staining was identified in the parietal epithelium of Bowman's capsule, distal convoluted tubule and collecting duct. Cellular and tight junction localization of claudin staining in renal tubules was heterogeneous and is discussed.

Conclusions: complex variation in the expression of human claudins likely determines paracellular permeability in the kidney. Altered claudin expression may influence pathologies involving abnormalities of absorption
claudins, glycolmethacrylate, paracellular permeability, renal tubule, tight junctions
0931-0509
2107-2119
Kirk, Adam
9c1e0bbe-06c2-4da9-aa19-e36b7a4d7f0f
Campbell, Sara
79c6d7a5-8b90-4e89-83b1-519a529b5eeb
Bass, Paul
1254f1dd-0239-4ebf-9ec1-50fed354e1a6
Mason, Juan
3100af92-384e-44ff-ad79-02ba359b9bcf
Collins, Jane
be0e66f1-3036-47fa-9d7e-914c48710ba4
Kirk, Adam
9c1e0bbe-06c2-4da9-aa19-e36b7a4d7f0f
Campbell, Sara
79c6d7a5-8b90-4e89-83b1-519a529b5eeb
Bass, Paul
1254f1dd-0239-4ebf-9ec1-50fed354e1a6
Mason, Juan
3100af92-384e-44ff-ad79-02ba359b9bcf
Collins, Jane
be0e66f1-3036-47fa-9d7e-914c48710ba4

Kirk, Adam, Campbell, Sara, Bass, Paul, Mason, Juan and Collins, Jane (2010) Differential expression of claudin tight junction proteins in the human cortical nephron. Nephrology, Dialysis, Transplantation, 25 (7), 2107-2119. (doi:10.1093/ndt/gfq006).

Record type: Article

Abstract

Background: in renal tubules, paracellular permeability is tightly controlled to facilitate solute absorption and urinary concentration and is regulated by tight junctions, which incorporate claudin proteins. There is very limited information confirming the localization of these proteins in the human renal cortex. Most data is inferred from mouse, bovine and rabbit studies and differences exist between mouse and other species.

Methods: a survey of claudin staining was performed on human kidney cortex embedded in glycolmethacrylate resin to enhance tissue morphology and facilitate the cutting of 2 µm serial sections.

Results: claudin-2, -10 and -11 antibodies labelled renal tubular epithelial cells, correlating with Lotus tetragonolobus and N-cadherin positive proximal tubules. Claudin-3, -10, -11 and -16 antibodies strongly stained a population of tubules that were positive for Tamm Horsfall protein on adjacent sections, confirming expression in the thick ascending limb of the Loop of Henle. Claudin-3, -4 and -8 antibodies reacted with tubules that correlated with the distal nephron markers, E-cadherin, epithelial membrane antigen and Dolichos biflorus and claudin-3, -4, -7 and -8 with the distal tubule marker, calbindin, and the collecting duct marker, aquaporin-2. Claudin-14 was localized in distal convoluted tubules, correlating positively with calbindin but negatively with aquaporin-2, whereas claudin-1 staining was identified in the parietal epithelium of Bowman's capsule, distal convoluted tubule and collecting duct. Cellular and tight junction localization of claudin staining in renal tubules was heterogeneous and is discussed.

Conclusions: complex variation in the expression of human claudins likely determines paracellular permeability in the kidney. Altered claudin expression may influence pathologies involving abnormalities of absorption

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More information

Submitted date: 2010
Accepted/In Press date: 2010
Published date: July 2010
Keywords: claudins, glycolmethacrylate, paracellular permeability, renal tubule, tight junctions
Organisations: Infection Inflammation & Immunity

Identifiers

Local EPrints ID: 73030
URI: http://eprints.soton.ac.uk/id/eprint/73030
ISSN: 0931-0509
PURE UUID: 3a240fe2-0420-42b5-8b00-58fb6bfb6114

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Date deposited: 26 Feb 2010
Last modified: 13 Mar 2024 21:49

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Contributors

Author: Adam Kirk
Author: Sara Campbell
Author: Paul Bass
Author: Juan Mason
Author: Jane Collins

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