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Detection and quantification of thrombomodulin in human semen

Detection and quantification of thrombomodulin in human semen
Detection and quantification of thrombomodulin in human semen
The presence of fibrin degradation products, thrombin-like enzyme, prothrombin fragments, thrombin-activatable fibrinolysis inhibitor, plasmin and other active components of blood coagulation and fibrinolysis in seminal plasma has been reported. In the present study we investigate the presence of thrombomodulin in human semen. Using an Imubind thrombomodulin enzyme-linked immunosorbent assay (American Diagnostica Inc., Stamford, Connecticut, USA), seminal thrombomodulin levels were measured in 47 semen specimens obtained from subfertile individuals, normally fertile individuals, semen donors as well as vasectomized individuals, and in a further group defined by normality in several parameters derived from the World Health Organization fertility criteria. Conventional semen parameters were analysed in all semen samples. Thrombomodulin is quantifiable in human semen at a concentration lower than that normally found in citrated blood plasma samples. Slightly higher levels were seen for fertile stratifications compared with infertile individuals but without significant difference, given the numbers accrued. A vasectomized group showed the lowest value. In conclusion, our results establish the presence of thrombomodulin in human semen and suggest its production both upstream and downstream from the level of a vasectomy lesion.
0957-5235
142-145
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Greenfield, Robert S.
afd44cc4-2a6b-41cc-b2fa-27d25212db5f
Cooper, Alan J.
65dcd1e1-3fcd-46b8-ad5f-f17e0d5b80a5
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Greenfield, Robert S.
afd44cc4-2a6b-41cc-b2fa-27d25212db5f
Cooper, Alan J.
65dcd1e1-3fcd-46b8-ad5f-f17e0d5b80a5

Lwaleed, Bashir A., Greenfield, Robert S. and Cooper, Alan J. (2008) Detection and quantification of thrombomodulin in human semen. Blood Coagulation & Fibrinolysis, 19 (2), 142-145. (doi:10.1097/MBC.0b013e3282f44e54). (PMID:18277135)

Record type: Article

Abstract

The presence of fibrin degradation products, thrombin-like enzyme, prothrombin fragments, thrombin-activatable fibrinolysis inhibitor, plasmin and other active components of blood coagulation and fibrinolysis in seminal plasma has been reported. In the present study we investigate the presence of thrombomodulin in human semen. Using an Imubind thrombomodulin enzyme-linked immunosorbent assay (American Diagnostica Inc., Stamford, Connecticut, USA), seminal thrombomodulin levels were measured in 47 semen specimens obtained from subfertile individuals, normally fertile individuals, semen donors as well as vasectomized individuals, and in a further group defined by normality in several parameters derived from the World Health Organization fertility criteria. Conventional semen parameters were analysed in all semen samples. Thrombomodulin is quantifiable in human semen at a concentration lower than that normally found in citrated blood plasma samples. Slightly higher levels were seen for fertile stratifications compared with infertile individuals but without significant difference, given the numbers accrued. A vasectomized group showed the lowest value. In conclusion, our results establish the presence of thrombomodulin in human semen and suggest its production both upstream and downstream from the level of a vasectomy lesion.

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Published date: March 2008

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Local EPrints ID: 73035
URI: https://eprints.soton.ac.uk/id/eprint/73035
ISSN: 0957-5235
PURE UUID: 0ca74633-babc-4ffd-ac93-71556444cd10

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Date deposited: 26 Feb 2010
Last modified: 19 Jul 2019 23:42

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