NPY augments the proliferative effect of FGF2 and increases the expression of FGFR1 on nestin positive postnatal hippocampal precursor cells, via the Y1 receptor
NPY augments the proliferative effect of FGF2 and increases the expression of FGFR1 on nestin positive postnatal hippocampal precursor cells, via the Y1 receptor
We have shown that neuropeptide Y (NPY), a peptide neurotransmitter released by hippocampal interneurons, is proliferative for hippocampal neural stem progenitor cells (NSPCs) via the Y1 receptor. Fibroblast growth factor 2 (FGF2), released predominantly by astrocytes, is also a powerful mitogen for postnatal and adult NSPCs, via the FGFR1 receptor. Knockout studies show that NPY and FGF2 are individually necessary, but not sufficient, for seizure-induced neurogenesis, suggesting a possible interaction. Here we examined for interactions between NPY and FGF2 on NSPCs from the postnatal hippocampus and report that the combination of NPY and FGF2 significantly shortens the cell cycle time of nestin positive NSPCs, more than either factor alone. This augmentation of proliferation rate is NPY Y1 receptor mediated, and Y1 receptor activation increases both FGFR1 mRNA and protein in NSPC cultures. NSPCs immunostain for both Y1 and FGFR1 receptors and the interaction is specific for dentate NSPCs.
This is the first report of a proliferative factor that augments the proliferative effect of FGF2 and is the first evidence of a positive proliferative interaction between a glial growth factor and a neuronal transmitter, identifying a novel neural activity driven mechanism for modulating the proliferation of hippocampal NSPCs.
neuropeptide y, fibroblastic growth factor 2, prox-1, dentate, gyrus, neurogenesis
Rodrigo, Chamira
98039229-ce81-41aa-bc1c-f0103426fb9f
Zaben, Malik
6390c24c-4458-486d-9188-3afda5c91359
Lawrence, Timothy
4f3a3c7a-248c-4ad2-aa42-29a4254f970c
Laskowski, Alexandra
f06f47d0-8587-4074-91b6-eb59f28572ae
Howell, Owain W.
59b875da-844e-4738-9b3b-b9796cf3b21f
Gray, William P.
f34a0e23-3cba-4b0a-8676-a1b2c3e4c095
1 February 2010
Rodrigo, Chamira
98039229-ce81-41aa-bc1c-f0103426fb9f
Zaben, Malik
6390c24c-4458-486d-9188-3afda5c91359
Lawrence, Timothy
4f3a3c7a-248c-4ad2-aa42-29a4254f970c
Laskowski, Alexandra
f06f47d0-8587-4074-91b6-eb59f28572ae
Howell, Owain W.
59b875da-844e-4738-9b3b-b9796cf3b21f
Gray, William P.
f34a0e23-3cba-4b0a-8676-a1b2c3e4c095
Rodrigo, Chamira, Zaben, Malik, Lawrence, Timothy, Laskowski, Alexandra, Howell, Owain W. and Gray, William P.
(2010)
NPY augments the proliferative effect of FGF2 and increases the expression of FGFR1 on nestin positive postnatal hippocampal precursor cells, via the Y1 receptor.
Journal of Neurochemistry.
(doi:10.1111/j.1471-4159.2010.06633.x).
Abstract
We have shown that neuropeptide Y (NPY), a peptide neurotransmitter released by hippocampal interneurons, is proliferative for hippocampal neural stem progenitor cells (NSPCs) via the Y1 receptor. Fibroblast growth factor 2 (FGF2), released predominantly by astrocytes, is also a powerful mitogen for postnatal and adult NSPCs, via the FGFR1 receptor. Knockout studies show that NPY and FGF2 are individually necessary, but not sufficient, for seizure-induced neurogenesis, suggesting a possible interaction. Here we examined for interactions between NPY and FGF2 on NSPCs from the postnatal hippocampus and report that the combination of NPY and FGF2 significantly shortens the cell cycle time of nestin positive NSPCs, more than either factor alone. This augmentation of proliferation rate is NPY Y1 receptor mediated, and Y1 receptor activation increases both FGFR1 mRNA and protein in NSPC cultures. NSPCs immunostain for both Y1 and FGFR1 receptors and the interaction is specific for dentate NSPCs.
This is the first report of a proliferative factor that augments the proliferative effect of FGF2 and is the first evidence of a positive proliferative interaction between a glial growth factor and a neuronal transmitter, identifying a novel neural activity driven mechanism for modulating the proliferation of hippocampal NSPCs.
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Published date: 1 February 2010
Keywords:
neuropeptide y, fibroblastic growth factor 2, prox-1, dentate, gyrus, neurogenesis
Identifiers
Local EPrints ID: 73268
URI: http://eprints.soton.ac.uk/id/eprint/73268
ISSN: 0022-3042
PURE UUID: 95cdb626-d437-4c61-bb54-f3d1695e1586
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Date deposited: 04 Mar 2010
Last modified: 13 Mar 2024 21:58
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Contributors
Author:
Chamira Rodrigo
Author:
Malik Zaben
Author:
Timothy Lawrence
Author:
Alexandra Laskowski
Author:
Owain W. Howell
Author:
William P. Gray
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